FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000736184> ?p ?o ?g. }

• W2000736184 endingPage "456" @default.
• W2000736184 startingPage "445" @default.
• W2000736184 abstract "5 min of tryptic digestion of purified rabbit skeletal α-actinin decreases by approximately 75% the ability of α-actinin to cross-link F-actin filaments as measured viscometrically at 27°C, but has little effect on the sedimentation coefficient of α-actinin at 20°C or on α-actinin's ability to increase the Mg2+-modified ATPase activity and rate of turbidity increase of reconstituted actomyosin suspensions. Twenty to sixty min of trypsin treatment reduces the sedimentation coefficient of α-actinin and destroys much of α-actinin's ability to increase the Mg2+-modified ATPase and rate of turbidity increase of reconstituted actomyosin suspensions. Therefore the ability of α-actinin to increase the rate of in vitro measures of muscle contraction may not result directly from α-actinin's ability to cross-link F-actin filaments. Trypsin does not split α-actinin into large fragments as it does myosin. Previous studies have shown that 35 to 65% of total tryptic-susceptible peptide bonds in α-actinin are split after 60 min of incubation with trypsin and that 30% of these bonds split in 60 min are cleaved during the first 5 min in a rapid reaction. That splitting of this group of peptide bonds has little effect on the sedimentation coefficient of α-actinin indicates that these bonds are located in a region of the α-actinin molecule where noncovalent forces are strong enough to maintain conformation of the native α-actinin molecule even after these bonds have been split. This ostensible segregation of α-actinin's ability to cross-link F-actin filaments from its ability to increase rate of in vitro assays of contraction by tryptic digestion may suggest that α-actinin could have at least two different physiological roles: (1) to bind actin filaments to each other or to basal structures, and (2) to enhance the effectiveness of actin in supporting movement." @default.
• W2000736184 created "2016-06-24" @default.
• W2000736184 creator A5008513659 @default.
• W2000736184 creator A5012087403 @default.
• W2000736184 creator A5023436601 @default.
• W2000736184 creator A5023528630 @default.
• W2000736184 creator A5048245933 @default.
• W2000736184 date "1976-10-01" @default.
• W2000736184 modified "2023-09-27" @default.
• W2000736184 title "Effect of trypsin on rabbit skeletal muscle α-actinin" @default.
• W2000736184 cites W1481017682 @default.
• W2000736184 cites W1490802671 @default.
• W2000736184 cites W1506043065 @default.
• W2000736184 cites W1521392208 @default.
• W2000736184 cites W1533173993 @default.
• W2000736184 cites W1555213420 @default.
• W2000736184 cites W1569313009 @default.
• W2000736184 cites W1581804688 @default.
• W2000736184 cites W1594703227 @default.
• W2000736184 cites W1625660792 @default.
• W2000736184 cites W1817530345 @default.
• W2000736184 cites W1914128339 @default.
• W2000736184 cites W1971446411 @default.
• W2000736184 cites W1992023596 @default.
• W2000736184 cites W1995855904 @default.
• W2000736184 cites W2024499829 @default.
• W2000736184 cites W2036775650 @default.
• W2000736184 cites W2039855901 @default.
• W2000736184 cites W2042006907 @default.
• W2000736184 cites W2047740472 @default.
• W2000736184 cites W2048553466 @default.
• W2000736184 cites W2050609889 @default.
• W2000736184 cites W2054716663 @default.
• W2000736184 cites W2067044612 @default.
• W2000736184 cites W2073101965 @default.
• W2000736184 cites W2082969032 @default.
• W2000736184 cites W2418850862 @default.
• W2000736184 cites W259174617 @default.
• W2000736184 cites W63309837 @default.
• W2000736184 doi "https://doi.org/10.1016/0005-2795(76)90010-6" @default.
• W2000736184 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/990286" @default.
• W2000736184 hasPublicationYear "1976" @default.
• W2000736184 type Work @default.
• W2000736184 sameAs 2000736184 @default.
• W2000736184 citedByCount "5" @default.
• W2000736184 countsByYear W20007361842023 @default.
• W2000736184 crossrefType "journal-article" @default.
• W2000736184 hasAuthorship W2000736184A5008513659 @default.
• W2000736184 hasAuthorship W2000736184A5012087403 @default.
• W2000736184 hasAuthorship W2000736184A5023436601 @default.
• W2000736184 hasAuthorship W2000736184A5023528630 @default.
• W2000736184 hasAuthorship W2000736184A5048245933 @default.
• W2000736184 hasConcept C12554922 @default.
• W2000736184 hasConcept C125705527 @default.
• W2000736184 hasConcept C133702464 @default.
• W2000736184 hasConcept C1388644 @default.
• W2000736184 hasConcept C142669718 @default.
• W2000736184 hasConcept C1491633281 @default.
• W2000736184 hasConcept C181199279 @default.
• W2000736184 hasConcept C185592680 @default.
• W2000736184 hasConcept C202751555 @default.
• W2000736184 hasConcept C2780340462 @default.
• W2000736184 hasConcept C55493867 @default.
• W2000736184 hasConcept C6997183 @default.
• W2000736184 hasConcept C86803240 @default.
• W2000736184 hasConceptScore W2000736184C12554922 @default.
• W2000736184 hasConceptScore W2000736184C125705527 @default.
• W2000736184 hasConceptScore W2000736184C133702464 @default.
• W2000736184 hasConceptScore W2000736184C1388644 @default.
• W2000736184 hasConceptScore W2000736184C142669718 @default.
• W2000736184 hasConceptScore W2000736184C1491633281 @default.
• W2000736184 hasConceptScore W2000736184C181199279 @default.
• W2000736184 hasConceptScore W2000736184C185592680 @default.
• W2000736184 hasConceptScore W2000736184C202751555 @default.
• W2000736184 hasConceptScore W2000736184C2780340462 @default.
• W2000736184 hasConceptScore W2000736184C55493867 @default.
• W2000736184 hasConceptScore W2000736184C6997183 @default.
• W2000736184 hasConceptScore W2000736184C86803240 @default.
• W2000736184 hasIssue "2" @default.
• W2000736184 hasLocation W20007361841 @default.
• W2000736184 hasLocation W20007361842 @default.
• W2000736184 hasOpenAccess W2000736184 @default.
• W2000736184 hasPrimaryLocation W20007361841 @default.
• W2000736184 hasRelatedWork W110208742 @default.
• W2000736184 hasRelatedWork W1990410177 @default.
• W2000736184 hasRelatedWork W2028529336 @default.
• W2000736184 hasRelatedWork W2112114713 @default.
• W2000736184 hasRelatedWork W2113186347 @default.
• W2000736184 hasRelatedWork W2584251531 @default.
• W2000736184 hasRelatedWork W2613165074 @default.
• W2000736184 hasRelatedWork W4213456994 @default.
• W2000736184 hasRelatedWork W4308247217 @default.
• W2000736184 hasRelatedWork W4315754344 @default.
• W2000736184 hasVolume "446" @default.
• W2000736184 isParatext "false" @default.
• W2000736184 isRetracted "false" @default.
• W2000736184 magId "2000736184" @default.
• W2000736184 workType "article" @default.