Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000736206> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2000736206 endingPage "236" @default.
- W2000736206 startingPage "231" @default.
- W2000736206 abstract "Egg-laying hens are important candidate bioreactors for pharmaceutical protein production because of the amenability of their eggs for protein expression. In this study, we constructed an oviduct-specific vector containing tissue plasminogen activator (tPA) protein and green fluorescent protein (pL-2.8OVtPAGFP) and assessed its expression in vitro and in vivo. Oviduct epithelial and 3T3 cells were cultured and transfected with pL-2.8OVtPAGFP and pEGP-N1 (control vector), respectively. The pL-2.8OVtPAGFP vector was administered to laying hens via a wing vein and their eggs and tissues were examined for tPA expression. The oviduct-specific vector pL-2.8OVtPAGFP was expressed only in oviduct epithelial cells whereas pEGP-N1 was detected in oviduct epithelial and 3T3 cells. Western blotting detected a 89 kDa band corresponding to tPA in egg white and oviduct epithelial cells, thus confirming expression of the protein. The amount of tPAGFP in eggs ranged 9 to 41 ng/mL on the third day after vector injection. The tPA expressed in egg white and oviduct epithelial cells showed fibrinolytic activity, indicating that the protein was expressed in active form. GFP was observed only in oviducts, with no detection in heart, muscle, liver and intestine. This is the first study to report the expression of tPA in egg white and oviduct epithelial cells using an oviduct-specific vector." @default.
- W2000736206 created "2016-06-24" @default.
- W2000736206 creator A5051769296 @default.
- W2000736206 creator A5062922297 @default.
- W2000736206 creator A5082066685 @default.
- W2000736206 creator A5090351184 @default.
- W2000736206 date "2011-04-08" @default.
- W2000736206 modified "2023-10-16" @default.
- W2000736206 title "Oviduct-specific expression of tissue plasminogen activator in laying hens" @default.
- W2000736206 cites W1523022919 @default.
- W2000736206 cites W1967724255 @default.
- W2000736206 cites W1983821958 @default.
- W2000736206 cites W2000247736 @default.
- W2000736206 cites W2021274076 @default.
- W2000736206 cites W2033493060 @default.
- W2000736206 cites W2034682531 @default.
- W2000736206 cites W2036090633 @default.
- W2000736206 cites W2041527227 @default.
- W2000736206 cites W2046032485 @default.
- W2000736206 cites W2073787098 @default.
- W2000736206 cites W2078281648 @default.
- W2000736206 cites W2083979580 @default.
- W2000736206 cites W2096111232 @default.
- W2000736206 cites W2099927165 @default.
- W2000736206 cites W2107641570 @default.
- W2000736206 cites W2110191620 @default.
- W2000736206 cites W2113245717 @default.
- W2000736206 cites W2170832108 @default.
- W2000736206 cites W2368257154 @default.
- W2000736206 doi "https://doi.org/10.1590/s1415-47572011005000011" @default.
- W2000736206 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3115315" @default.
- W2000736206 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21734822" @default.
- W2000736206 hasPublicationYear "2011" @default.
- W2000736206 type Work @default.
- W2000736206 sameAs 2000736206 @default.
- W2000736206 citedByCount "5" @default.
- W2000736206 countsByYear W20007362062012 @default.
- W2000736206 countsByYear W20007362062017 @default.
- W2000736206 countsByYear W20007362062018 @default.
- W2000736206 countsByYear W20007362062023 @default.
- W2000736206 crossrefType "journal-article" @default.
- W2000736206 hasAuthorship W2000736206A5051769296 @default.
- W2000736206 hasAuthorship W2000736206A5062922297 @default.
- W2000736206 hasAuthorship W2000736206A5082066685 @default.
- W2000736206 hasAuthorship W2000736206A5090351184 @default.
- W2000736206 hasBestOaLocation W20007362061 @default.
- W2000736206 hasConcept C104317684 @default.
- W2000736206 hasConcept C134018914 @default.
- W2000736206 hasConcept C153911025 @default.
- W2000736206 hasConcept C16685009 @default.
- W2000736206 hasConcept C2777129507 @default.
- W2000736206 hasConcept C2778484053 @default.
- W2000736206 hasConcept C2779679481 @default.
- W2000736206 hasConcept C33161422 @default.
- W2000736206 hasConcept C40767141 @default.
- W2000736206 hasConcept C54009773 @default.
- W2000736206 hasConcept C55493867 @default.
- W2000736206 hasConcept C71924100 @default.
- W2000736206 hasConcept C86803240 @default.
- W2000736206 hasConcept C88045685 @default.
- W2000736206 hasConceptScore W2000736206C104317684 @default.
- W2000736206 hasConceptScore W2000736206C134018914 @default.
- W2000736206 hasConceptScore W2000736206C153911025 @default.
- W2000736206 hasConceptScore W2000736206C16685009 @default.
- W2000736206 hasConceptScore W2000736206C2777129507 @default.
- W2000736206 hasConceptScore W2000736206C2778484053 @default.
- W2000736206 hasConceptScore W2000736206C2779679481 @default.
- W2000736206 hasConceptScore W2000736206C33161422 @default.
- W2000736206 hasConceptScore W2000736206C40767141 @default.
- W2000736206 hasConceptScore W2000736206C54009773 @default.
- W2000736206 hasConceptScore W2000736206C55493867 @default.
- W2000736206 hasConceptScore W2000736206C71924100 @default.
- W2000736206 hasConceptScore W2000736206C86803240 @default.
- W2000736206 hasConceptScore W2000736206C88045685 @default.
- W2000736206 hasIssue "2" @default.
- W2000736206 hasLocation W20007362061 @default.
- W2000736206 hasLocation W20007362062 @default.
- W2000736206 hasLocation W20007362063 @default.
- W2000736206 hasLocation W20007362064 @default.
- W2000736206 hasLocation W20007362065 @default.
- W2000736206 hasLocation W20007362066 @default.
- W2000736206 hasOpenAccess W2000736206 @default.
- W2000736206 hasPrimaryLocation W20007362061 @default.
- W2000736206 hasRelatedWork W2356208666 @default.
- W2000736206 hasRelatedWork W2375527106 @default.
- W2000736206 hasRelatedWork W2376443560 @default.
- W2000736206 hasRelatedWork W2387734610 @default.
- W2000736206 hasRelatedWork W2387801567 @default.
- W2000736206 hasRelatedWork W2390284118 @default.
- W2000736206 hasRelatedWork W2413079405 @default.
- W2000736206 hasRelatedWork W2421715018 @default.
- W2000736206 hasRelatedWork W2748409821 @default.
- W2000736206 hasRelatedWork W3025999129 @default.
- W2000736206 hasVolume "34" @default.
- W2000736206 isParatext "false" @default.
- W2000736206 isRetracted "false" @default.
- W2000736206 magId "2000736206" @default.
- W2000736206 workType "article" @default.