FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000736584> ?p ?o ?g. }

• W2000736584 endingPage "26580" @default.
• W2000736584 startingPage "26573" @default.
• W2000736584 abstract "The glucocorticoid receptor (GR) is phosphorylated at multiple serine residues in a hormone-dependent manner, yet progress on elucidating the function of GR phosphorylation has been hindered by the lack of a simple assay to detect receptor phosphorylation <i>in vivo</i>. We have produced antibodies that specifically recognize phosphorylation sites within human GR at Ser²⁰³ and Ser²¹¹. In the absence of hormone, the level of GR phosphorylation at Ser²¹¹ was low compared with phosphorylation at Ser²⁰³. Phosphorylation of both residues increased upon treatment with the GR agonist dexamethasone. Using a battery of agonists and antagonists, we found that the transcriptional activity of GR correlated with the amount of phosphorylation at Ser²¹¹, suggesting that Ser²¹¹ phosphorylation is a biomarker for activated GR <i>in vivo</i>. Mechanistically, the kinetics of Ser²⁰³ and Ser²¹¹ phosphorylation in response to hormone differed, with Ser²¹¹ displaying a more robust and sustained phosphorylation relative to Ser²⁰³. Analysis of GR immunoprecipitates with phospho-GR-specific antibodies indicated that the receptor was phosphorylated heterogeneously at Ser²⁰³ in the absence of hormone, whereas in the presence of hormone, a subpopulation of receptors was phosphorylated at both Ser²⁰³ and Ser²¹¹. Interestingly, biochemical fractionation studies following hormone treatment indicated that the Ser²⁰³-phosphorylated form of the receptor was predominantly cytoplasmic, whereas Ser²¹¹-phosphorylated GR was found in the nucleus. Likewise, by immunofluorescence, Ser²⁰³-phosphorylated GR was located in the cytoplasm and perinuclear regions of the cell, but not in the nucleoplasm, whereas strong phospho-Ser²¹¹ staining was evident in the nucleoplasm of hormone-treated cells. Our results suggest that differentially phosphorylated receptor species are located in unique subcellular compartments, likely modulating distinct aspects of receptor function." @default.
• W2000736584 created "2016-06-24" @default.
• W2000736584 creator A5029817264 @default.
• W2000736584 creator A5060305478 @default.
• W2000736584 creator A5065058975 @default.
• W2000736584 date "2002-07-01" @default.
• W2000736584 modified "2023-10-14" @default.
• W2000736584 title "Deciphering the Phosphorylation “Code” of the Glucocorticoid Receptor in Vivo" @default.
• W2000736584 cites W1479704824 @default.
• W2000736584 cites W1565210265 @default.
• W2000736584 cites W1582124530 @default.
• W2000736584 cites W1597897944 @default.
• W2000736584 cites W1961241993 @default.
• W2000736584 cites W1963612767 @default.
• W2000736584 cites W1967169977 @default.
• W2000736584 cites W1967966561 @default.
• W2000736584 cites W1974334424 @default.
• W2000736584 cites W1980292048 @default.
• W2000736584 cites W1990379626 @default.
• W2000736584 cites W1992515328 @default.
• W2000736584 cites W1999718274 @default.
• W2000736584 cites W2003934306 @default.
• W2000736584 cites W2007169317 @default.
• W2000736584 cites W2018095466 @default.
• W2000736584 cites W2030695420 @default.
• W2000736584 cites W2050403670 @default.
• W2000736584 cites W2051894528 @default.
• W2000736584 cites W2054306576 @default.
• W2000736584 cites W2074049733 @default.
• W2000736584 cites W2090604572 @default.
• W2000736584 cites W2102824660 @default.
• W2000736584 cites W2104360623 @default.
• W2000736584 cites W2121666098 @default.
• W2000736584 cites W2121745326 @default.
• W2000736584 cites W8163034 @default.
• W2000736584 doi "https://doi.org/10.1074/jbc.m110530200" @default.
• W2000736584 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12000743" @default.
• W2000736584 hasPublicationYear "2002" @default.
• W2000736584 type Work @default.
• W2000736584 sameAs 2000736584 @default.
• W2000736584 citedByCount "283" @default.
• W2000736584 countsByYear W20007365842012 @default.
• W2000736584 countsByYear W20007365842013 @default.
• W2000736584 countsByYear W20007365842014 @default.
• W2000736584 countsByYear W20007365842015 @default.
• W2000736584 countsByYear W20007365842016 @default.
• W2000736584 countsByYear W20007365842017 @default.
• W2000736584 countsByYear W20007365842018 @default.
• W2000736584 countsByYear W20007365842019 @default.
• W2000736584 countsByYear W20007365842020 @default.
• W2000736584 countsByYear W20007365842021 @default.
• W2000736584 countsByYear W20007365842022 @default.
• W2000736584 countsByYear W20007365842023 @default.
• W2000736584 crossrefType "journal-article" @default.
• W2000736584 hasAuthorship W2000736584A5029817264 @default.
• W2000736584 hasAuthorship W2000736584A5060305478 @default.
• W2000736584 hasAuthorship W2000736584A5065058975 @default.
• W2000736584 hasBestOaLocation W20007365841 @default.
• W2000736584 hasConcept C11960822 @default.
• W2000736584 hasConcept C153911025 @default.
• W2000736584 hasConcept C170493617 @default.
• W2000736584 hasConcept C185592680 @default.
• W2000736584 hasConcept C2776414213 @default.
• W2000736584 hasConcept C55493867 @default.
• W2000736584 hasConcept C59493245 @default.
• W2000736584 hasConcept C86803240 @default.
• W2000736584 hasConceptScore W2000736584C11960822 @default.
• W2000736584 hasConceptScore W2000736584C153911025 @default.
• W2000736584 hasConceptScore W2000736584C170493617 @default.
• W2000736584 hasConceptScore W2000736584C185592680 @default.
• W2000736584 hasConceptScore W2000736584C2776414213 @default.
• W2000736584 hasConceptScore W2000736584C55493867 @default.
• W2000736584 hasConceptScore W2000736584C59493245 @default.
• W2000736584 hasConceptScore W2000736584C86803240 @default.
• W2000736584 hasIssue "29" @default.
• W2000736584 hasLocation W20007365841 @default.
• W2000736584 hasOpenAccess W2000736584 @default.
• W2000736584 hasPrimaryLocation W20007365841 @default.
• W2000736584 hasRelatedWork W1953044259 @default.
• W2000736584 hasRelatedWork W2049037526 @default.
• W2000736584 hasRelatedWork W2109572947 @default.
• W2000736584 hasRelatedWork W2162564135 @default.
• W2000736584 hasRelatedWork W2332847116 @default.
• W2000736584 hasRelatedWork W2888018387 @default.
• W2000736584 hasRelatedWork W2915211422 @default.
• W2000736584 hasRelatedWork W3038048989 @default.
• W2000736584 hasRelatedWork W615169925 @default.
• W2000736584 hasRelatedWork W2136545008 @default.
• W2000736584 hasVolume "277" @default.
• W2000736584 isParatext "false" @default.
• W2000736584 isRetracted "false" @default.
• W2000736584 magId "2000736584" @default.
• W2000736584 workType "article" @default.