Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000738240> ?p ?o ?g. }
- W2000738240 endingPage "276" @default.
- W2000738240 startingPage "269" @default.
- W2000738240 abstract "Repetitive hormone-induced changes in concentration of free cytoplasmic Ca2+ in hepatocytes require Ca2+ entry through receptor-activated Ca2+ channels and SOCs (store-operated Ca2+ channels). SOCs are activated by a decrease in Ca2+ concentration in the intracellular Ca2+ stores, but the molecular components and mechanisms are not well understood. Some studies with other cell types suggest that PLC-γ (phospholipase C-γ) is involved in the activation of receptor-activated Ca2+ channels and/or SOCs, independently of PLC-γ-mediated generation of IP3 (inositol 1,4,5-trisphosphate). The nature of the Ca2+ channels regulated by PLC-γ has not been defined clearly. The aim of the present study was to determine if PLC-γ is required for the activation of SOCs in liver cells. Transfection of H4IIE cells derived from rat hepatocytes with siRNA (short interfering RNA) targeted to PLC-γ1 caused a reduction (by approx. 70%) in the PLC-γ1 protein expression, with maximal effect at 72–96 h. This was associated with a decrease (by approx. 60%) in the amplitude of the ISOC (store-operated Ca2+ current) developed in response to intracellular perfusion with either IP3 or thapsigargin. Knockdown of STIM1 (stromal interaction molecule type 1) by siRNA also resulted in a significant reduction (approx. 80% at 72 h post-transfection) of the ISOC amplitude. Immunoprecipitation of PLC-γ1 and STIM1, however, suggested that under the experimental conditions these proteins do not interact with each other. It is concluded that the PLC-γ1 protein, independently of IP3 generation and STIM1, is required to couple endoplasmic reticulum Ca2+ release to the activation of SOCs in the plasma membrane of H4IIE liver cells." @default.
- W2000738240 created "2016-06-24" @default.
- W2000738240 creator A5001046498 @default.
- W2000738240 creator A5004368460 @default.
- W2000738240 creator A5010801477 @default.
- W2000738240 creator A5015338055 @default.
- W2000738240 creator A5037220260 @default.
- W2000738240 creator A5051888301 @default.
- W2000738240 creator A5055432522 @default.
- W2000738240 date "2007-06-27" @default.
- W2000738240 modified "2023-10-12" @default.
- W2000738240 title "Phospholipase C-γ1 is required for the activation of store-operated Ca2+ channels in liver cells" @default.
- W2000738240 cites W1558947364 @default.
- W2000738240 cites W1967867298 @default.
- W2000738240 cites W1976584289 @default.
- W2000738240 cites W1978115491 @default.
- W2000738240 cites W1996024251 @default.
- W2000738240 cites W2000702625 @default.
- W2000738240 cites W2004670453 @default.
- W2000738240 cites W2005413789 @default.
- W2000738240 cites W2012471725 @default.
- W2000738240 cites W2013491384 @default.
- W2000738240 cites W2019235375 @default.
- W2000738240 cites W2020708758 @default.
- W2000738240 cites W2033452983 @default.
- W2000738240 cites W2042342392 @default.
- W2000738240 cites W2043217287 @default.
- W2000738240 cites W2052082800 @default.
- W2000738240 cites W2055048326 @default.
- W2000738240 cites W2060325331 @default.
- W2000738240 cites W2085475071 @default.
- W2000738240 cites W2087104781 @default.
- W2000738240 cites W2090018138 @default.
- W2000738240 cites W2091066571 @default.
- W2000738240 cites W2096549360 @default.
- W2000738240 cites W2097265484 @default.
- W2000738240 cites W2101586949 @default.
- W2000738240 cites W2133615985 @default.
- W2000738240 cites W2134597348 @default.
- W2000738240 cites W2137758625 @default.
- W2000738240 cites W2138751131 @default.
- W2000738240 cites W2139679033 @default.
- W2000738240 cites W2141260882 @default.
- W2000738240 cites W2156783552 @default.
- W2000738240 cites W2166802510 @default.
- W2000738240 cites W2171553829 @default.
- W2000738240 cites W4211039187 @default.
- W2000738240 cites W4248092016 @default.
- W2000738240 doi "https://doi.org/10.1042/bj20061762" @default.
- W2000738240 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1904522" @default.
- W2000738240 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17432954" @default.
- W2000738240 hasPublicationYear "2007" @default.
- W2000738240 type Work @default.
- W2000738240 sameAs 2000738240 @default.
- W2000738240 citedByCount "34" @default.
- W2000738240 countsByYear W20007382402012 @default.
- W2000738240 countsByYear W20007382402014 @default.
- W2000738240 countsByYear W20007382402015 @default.
- W2000738240 countsByYear W20007382402016 @default.
- W2000738240 countsByYear W20007382402017 @default.
- W2000738240 countsByYear W20007382402018 @default.
- W2000738240 countsByYear W20007382402019 @default.
- W2000738240 countsByYear W20007382402020 @default.
- W2000738240 crossrefType "journal-article" @default.
- W2000738240 hasAuthorship W2000738240A5001046498 @default.
- W2000738240 hasAuthorship W2000738240A5004368460 @default.
- W2000738240 hasAuthorship W2000738240A5010801477 @default.
- W2000738240 hasAuthorship W2000738240A5015338055 @default.
- W2000738240 hasAuthorship W2000738240A5037220260 @default.
- W2000738240 hasAuthorship W2000738240A5051888301 @default.
- W2000738240 hasAuthorship W2000738240A5055432522 @default.
- W2000738240 hasBestOaLocation W20007382402 @default.
- W2000738240 hasConcept C104317684 @default.
- W2000738240 hasConcept C152624196 @default.
- W2000738240 hasConcept C153911025 @default.
- W2000738240 hasConcept C158617107 @default.
- W2000738240 hasConcept C162740809 @default.
- W2000738240 hasConcept C170493617 @default.
- W2000738240 hasConcept C2776610495 @default.
- W2000738240 hasConcept C2777146706 @default.
- W2000738240 hasConcept C2777427919 @default.
- W2000738240 hasConcept C2778597717 @default.
- W2000738240 hasConcept C54009773 @default.
- W2000738240 hasConcept C55493867 @default.
- W2000738240 hasConcept C62478195 @default.
- W2000738240 hasConcept C71829478 @default.
- W2000738240 hasConcept C79879829 @default.
- W2000738240 hasConcept C86803240 @default.
- W2000738240 hasConcept C95444343 @default.
- W2000738240 hasConceptScore W2000738240C104317684 @default.
- W2000738240 hasConceptScore W2000738240C152624196 @default.
- W2000738240 hasConceptScore W2000738240C153911025 @default.
- W2000738240 hasConceptScore W2000738240C158617107 @default.
- W2000738240 hasConceptScore W2000738240C162740809 @default.
- W2000738240 hasConceptScore W2000738240C170493617 @default.
- W2000738240 hasConceptScore W2000738240C2776610495 @default.
- W2000738240 hasConceptScore W2000738240C2777146706 @default.
- W2000738240 hasConceptScore W2000738240C2777427919 @default.