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• W2000743510 abstract "The Graffi murine leukemia virus (MuLV) is a retroviral mixture that induces predominantly myeloid leukemia in several inbred strains of mice. To analyze the viral component responsible for the myeloid leukemogenesis, we cloned several proviruses from a Graffi MuLV-infected cell line. Several infectious molecular clones were obtained that could be classified into two distinct groups of infectious MuLV. Both types of MuLV were nondefective, ecotropic, and NB tropic and induced granulocytic leukemia in BALB/c and NFS mice. Restriction enzyme analysis and molecular hybridization with several MuLV probes on one molecular clone from each group revealed that both groups are closely related to each other but are clearly distinct from all known retroviruses. One component of MuLV, however, induced leukemia with a shorter latency period and harbored a lengthier long terminal repeat. The long terminal repeat of the more leukemogenic component of MuLV had acquired a 60-bp perfect duplication in the U3 region. Analysis of the tumor DNAs with probes for the mouse T-cell receptor and immunoglobulin heavy chain genes revealed frequent rearrangements with one or both probes. This concomitant expression by leukemic cells of markers of different lineages, observed in human leukemias, has been termed lineage infidelity and confirms that the latter rearrangements are not restricted to hematopoietic precursors committed to lymphoid differentiation." @default.
• W2000743510 created "2016-06-24" @default.
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• W2000743510 date "1993-08-01" @default.
• W2000743510 modified "2023-10-01" @default.
• W2000743510 title "Graffi murine leukemia virus: molecular cloning and characterization of the myeloid leukemia-inducing agent" @default.
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• W2000743510 doi "https://doi.org/10.1128/jvi.67.8.4722-4731.1993" @default.
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