Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000744455> ?p ?o ?g. }
- W2000744455 endingPage "506" @default.
- W2000744455 startingPage "499" @default.
- W2000744455 abstract "Cathepsin K is known to play an important role in bone resorption, and it has the P2 specificity for proline. Rat cathepsin K has 88% identity with the human enzyme. However, it has been reported that its enzymatic activity for a Cbz-Leu-Arg-MCA substrate is lower than that of human cathepsin K, and that the rat enzyme is not well inhibited by human cathepsin K inhibitors. For this study, we prepared recombinant enzyme to investigate the substrate specificity of rat cathepsin K. Cleavage experiments using the fragment of type I collagen and peptidic libraries demonstrated that rat cathepsin K preferentially hydrolyses the substrates at the P2 Hyp position. Comparison of the S2 site between rat and human cathepsin K sequences indicated that two S2 residues at Ser134 and Val160 in rat are varied to Ala and Leu, respectively, in the human enzyme. Cleavage experiments using two single mutants, S134A and V160L, and one double mutant, S134A/V160L, of rat cathepsin K showed that all the rat mutants lost the P2 Hyp specificity. The information obtained from our comparative studies on rat and human cathepsin K should make a significant impact on developing specific inhibitors of human cathepsin K since rat is usually used as test species." @default.
- W2000744455 created "2016-06-24" @default.
- W2000744455 creator A5009197517 @default.
- W2000744455 creator A5009468301 @default.
- W2000744455 creator A5034188197 @default.
- W2000744455 creator A5044738843 @default.
- W2000744455 creator A5061327754 @default.
- W2000744455 creator A5088854730 @default.
- W2000744455 date "2008-06-16" @default.
- W2000744455 modified "2023-10-16" @default.
- W2000744455 title "Species Differences Between Human and Rat in the Substrate Specificity of Cathepsin K" @default.
- W2000744455 cites W1515227258 @default.
- W2000744455 cites W1579781544 @default.
- W2000744455 cites W1583830460 @default.
- W2000744455 cites W1593295279 @default.
- W2000744455 cites W1762318613 @default.
- W2000744455 cites W1856801024 @default.
- W2000744455 cites W1894541755 @default.
- W2000744455 cites W1964094449 @default.
- W2000744455 cites W1968024679 @default.
- W2000744455 cites W1973334154 @default.
- W2000744455 cites W1978181825 @default.
- W2000744455 cites W1981577989 @default.
- W2000744455 cites W1984965767 @default.
- W2000744455 cites W2009672068 @default.
- W2000744455 cites W2025062557 @default.
- W2000744455 cites W2029347297 @default.
- W2000744455 cites W2036300408 @default.
- W2000744455 cites W2038114059 @default.
- W2000744455 cites W2043068076 @default.
- W2000744455 cites W2047214620 @default.
- W2000744455 cites W2049528994 @default.
- W2000744455 cites W2050513051 @default.
- W2000744455 cites W2058616210 @default.
- W2000744455 cites W2059018472 @default.
- W2000744455 cites W2069051214 @default.
- W2000744455 cites W2070195347 @default.
- W2000744455 cites W2073485842 @default.
- W2000744455 cites W2078146467 @default.
- W2000744455 cites W2083294270 @default.
- W2000744455 cites W2090177025 @default.
- W2000744455 cites W2090299301 @default.
- W2000744455 cites W2100359805 @default.
- W2000744455 cites W2110236793 @default.
- W2000744455 cites W2110247426 @default.
- W2000744455 cites W2124507977 @default.
- W2000744455 cites W2153462664 @default.
- W2000744455 cites W2153686427 @default.
- W2000744455 cites W2160174421 @default.
- W2000744455 cites W2161115679 @default.
- W2000744455 cites W2333911447 @default.
- W2000744455 doi "https://doi.org/10.1093/jb/mvn093" @default.
- W2000744455 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18664521" @default.
- W2000744455 hasPublicationYear "2008" @default.
- W2000744455 type Work @default.
- W2000744455 sameAs 2000744455 @default.
- W2000744455 citedByCount "6" @default.
- W2000744455 countsByYear W20007444552015 @default.
- W2000744455 countsByYear W20007444552017 @default.
- W2000744455 countsByYear W20007444552020 @default.
- W2000744455 countsByYear W20007444552022 @default.
- W2000744455 crossrefType "journal-article" @default.
- W2000744455 hasAuthorship W2000744455A5009197517 @default.
- W2000744455 hasAuthorship W2000744455A5009468301 @default.
- W2000744455 hasAuthorship W2000744455A5034188197 @default.
- W2000744455 hasAuthorship W2000744455A5044738843 @default.
- W2000744455 hasAuthorship W2000744455A5061327754 @default.
- W2000744455 hasAuthorship W2000744455A5088854730 @default.
- W2000744455 hasConcept C104317684 @default.
- W2000744455 hasConcept C143065580 @default.
- W2000744455 hasConcept C145734084 @default.
- W2000744455 hasConcept C151730666 @default.
- W2000744455 hasConcept C153911025 @default.
- W2000744455 hasConcept C167844969 @default.
- W2000744455 hasConcept C173633252 @default.
- W2000744455 hasConcept C174937957 @default.
- W2000744455 hasConcept C175156509 @default.
- W2000744455 hasConcept C181199279 @default.
- W2000744455 hasConcept C185592680 @default.
- W2000744455 hasConcept C2780034444 @default.
- W2000744455 hasConcept C34695024 @default.
- W2000744455 hasConcept C43369102 @default.
- W2000744455 hasConcept C51618164 @default.
- W2000744455 hasConcept C55493867 @default.
- W2000744455 hasConcept C78553746 @default.
- W2000744455 hasConcept C81216549 @default.
- W2000744455 hasConcept C86803240 @default.
- W2000744455 hasConceptScore W2000744455C104317684 @default.
- W2000744455 hasConceptScore W2000744455C143065580 @default.
- W2000744455 hasConceptScore W2000744455C145734084 @default.
- W2000744455 hasConceptScore W2000744455C151730666 @default.
- W2000744455 hasConceptScore W2000744455C153911025 @default.
- W2000744455 hasConceptScore W2000744455C167844969 @default.
- W2000744455 hasConceptScore W2000744455C173633252 @default.
- W2000744455 hasConceptScore W2000744455C174937957 @default.
- W2000744455 hasConceptScore W2000744455C175156509 @default.
- W2000744455 hasConceptScore W2000744455C181199279 @default.
- W2000744455 hasConceptScore W2000744455C185592680 @default.