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- W2000744761 abstract "The reaction of histone F1 with tetranitromethane was used to study the environment of the single tyrosine residue in the molecule. It was found that at a 10-fold molar excess of tetranitromethane over tyrosine approx. 40 min were needed to convert 85% of the tyrosine residues to nitrotyrosine. The rate and extent of nitration of F1 in dilute salt solutions was used as a reference against which the rate and extent of nitration under conditions known to affect the conformation of F1 was measured. In 1.0 M NaCl solutions the rate of nitration decreased, suggesting that the presence of salt may cause a conformational change in the tyrosine containing region of the histone. When the histone is complexed with polyanions such as DNA, RNA or poly-L-glutamic acid the accessibility of the tyrosine to nitration is markedly reduced. The results indicate that upon association with polyanions the non-cationic, tyrosine-containing, region in F1 undergoes a conformational change or alternatively, this tyrosine containing region is tightly associated with the polyanion." @default.
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- W2000744761 date "1971-11-01" @default.
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- W2000744761 title "Nitration of the tyrosine in histone F1 in salt solutions and in F1-polyanion complexes" @default.
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- W2000744761 doi "https://doi.org/10.1016/0005-2795(71)90100-0" @default.
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