Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000752771> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2000752771 endingPage "914" @default.
- W2000752771 startingPage "901" @default.
- W2000752771 abstract "Bacteriophage φ29 DNA with covalently bound terminal protein (DNA-gp3) and its left and right-end restriction fragments (L and R-DNA-gp3) sedimented faster in sucrose density gradients than their proteinase K-treated counterparts, and the faster sedimentation was both gp3 and Mg2+-dependent. Addition of gp16, the φ29 DNA packaging ATPase, further increased the sedimentation rates of both intact DNA-gp3 and L and R-DNA-gp3 fragments. Thus, DNAs with gp3 were more compact than gp3-free DNA, and gp16 further condensed the DNA-gp3 forms. [35S]gp16 cosedimented with the fast-sedimenting DNA-gp3 fragments, and the putative L-DNA-gp3-gp16 complexes were packaged preferentially into proheads in the defined in vitro system. Lariats of DNA-gp3 and L and R-DNA-gp3 observed by electron microscopy rationalized the sedimentation results, and lariats with multiple loops or coils increased tenfold in a preparation of L-DNA-gp3-gp16 complexes. The rapid sedimentation and the structure of the DNA-gp3-gp16 complexes were consistent with supercoiling of lariat loops, and treatment with topoisomerase I shifted fast-sedimenting complexes toward the uncoiled lariat position in sucrose density gradients. DNA-gp3 has a maturation pathway in which the packaging proteins gp3 and gp16 supercoil the DNA ends, probably as a prerequisite for efficient interaction with the prohead." @default.
- W2000752771 created "2016-06-24" @default.
- W2000752771 creator A5054316342 @default.
- W2000752771 creator A5056217085 @default.
- W2000752771 date "1997-03-01" @default.
- W2000752771 modified "2023-10-14" @default.
- W2000752771 title "The bacteriophage φ29 packaging proteins supercoil the DNA ends" @default.
- W2000752771 cites W124504601 @default.
- W2000752771 cites W1436736130 @default.
- W2000752771 cites W1494512087 @default.
- W2000752771 cites W1565035996 @default.
- W2000752771 cites W169971741 @default.
- W2000752771 cites W1821288009 @default.
- W2000752771 cites W1957495451 @default.
- W2000752771 cites W1966384509 @default.
- W2000752771 cites W1971442611 @default.
- W2000752771 cites W1984262288 @default.
- W2000752771 cites W1994931793 @default.
- W2000752771 cites W1998635418 @default.
- W2000752771 cites W2000495393 @default.
- W2000752771 cites W2007497237 @default.
- W2000752771 cites W2008343552 @default.
- W2000752771 cites W2024741426 @default.
- W2000752771 cites W2026614206 @default.
- W2000752771 cites W2033087969 @default.
- W2000752771 cites W2040069810 @default.
- W2000752771 cites W2045911086 @default.
- W2000752771 cites W2053338980 @default.
- W2000752771 cites W2063702812 @default.
- W2000752771 cites W2071904027 @default.
- W2000752771 cites W2079273943 @default.
- W2000752771 cites W2079530502 @default.
- W2000752771 cites W2121813899 @default.
- W2000752771 cites W2134955152 @default.
- W2000752771 doi "https://doi.org/10.1006/jmbi.1996.0843" @default.
- W2000752771 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9086269" @default.
- W2000752771 hasPublicationYear "1997" @default.
- W2000752771 type Work @default.
- W2000752771 sameAs 2000752771 @default.
- W2000752771 citedByCount "71" @default.
- W2000752771 countsByYear W20007527712012 @default.
- W2000752771 countsByYear W20007527712013 @default.
- W2000752771 countsByYear W20007527712014 @default.
- W2000752771 countsByYear W20007527712015 @default.
- W2000752771 countsByYear W20007527712016 @default.
- W2000752771 countsByYear W20007527712018 @default.
- W2000752771 countsByYear W20007527712019 @default.
- W2000752771 countsByYear W20007527712020 @default.
- W2000752771 countsByYear W20007527712021 @default.
- W2000752771 crossrefType "journal-article" @default.
- W2000752771 hasAuthorship W2000752771A5054316342 @default.
- W2000752771 hasAuthorship W2000752771A5056217085 @default.
- W2000752771 hasConcept C104317684 @default.
- W2000752771 hasConcept C12554922 @default.
- W2000752771 hasConcept C153911025 @default.
- W2000752771 hasConcept C158456008 @default.
- W2000752771 hasConcept C185592680 @default.
- W2000752771 hasConcept C2776441376 @default.
- W2000752771 hasConcept C547475151 @default.
- W2000752771 hasConcept C552990157 @default.
- W2000752771 hasConcept C55493867 @default.
- W2000752771 hasConcept C73573662 @default.
- W2000752771 hasConcept C8010536 @default.
- W2000752771 hasConcept C86803240 @default.
- W2000752771 hasConceptScore W2000752771C104317684 @default.
- W2000752771 hasConceptScore W2000752771C12554922 @default.
- W2000752771 hasConceptScore W2000752771C153911025 @default.
- W2000752771 hasConceptScore W2000752771C158456008 @default.
- W2000752771 hasConceptScore W2000752771C185592680 @default.
- W2000752771 hasConceptScore W2000752771C2776441376 @default.
- W2000752771 hasConceptScore W2000752771C547475151 @default.
- W2000752771 hasConceptScore W2000752771C552990157 @default.
- W2000752771 hasConceptScore W2000752771C55493867 @default.
- W2000752771 hasConceptScore W2000752771C73573662 @default.
- W2000752771 hasConceptScore W2000752771C8010536 @default.
- W2000752771 hasConceptScore W2000752771C86803240 @default.
- W2000752771 hasIssue "5" @default.
- W2000752771 hasLocation W20007527711 @default.
- W2000752771 hasLocation W20007527712 @default.
- W2000752771 hasOpenAccess W2000752771 @default.
- W2000752771 hasPrimaryLocation W20007527711 @default.
- W2000752771 hasRelatedWork W1974470327 @default.
- W2000752771 hasRelatedWork W1990154423 @default.
- W2000752771 hasRelatedWork W2020684201 @default.
- W2000752771 hasRelatedWork W2081305361 @default.
- W2000752771 hasRelatedWork W2097697790 @default.
- W2000752771 hasRelatedWork W2145381472 @default.
- W2000752771 hasRelatedWork W2154195781 @default.
- W2000752771 hasRelatedWork W2740036970 @default.
- W2000752771 hasRelatedWork W3184763144 @default.
- W2000752771 hasRelatedWork W44616578 @default.
- W2000752771 hasVolume "266" @default.
- W2000752771 isParatext "false" @default.
- W2000752771 isRetracted "false" @default.
- W2000752771 magId "2000752771" @default.
- W2000752771 workType "article" @default.