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- W2000754643 abstract "Summary The genetic aspects of ori C replication initiation in Mycobacterium tuberculosis are largely unknown. A two‐step genetic screen was utilized for isolating M. tuberculosis dna A cold‐sensitive (cos) mutants. First, a resident plasmid expressing functional dna A integrated at the att B locus in dna A null background was exchanged with an incoming plasmid bearing a mutagenized dna A gene. Next, the mutants that were defective for growth at 30°C, a non‐permissive temperature, but resumed growth and DNA synthesis when shifted to 37°C, a permissive temperature, were subsequently selected. Nucleotide sequencing analysis located mutations to different regions of the dna A gene. Modulation of the growth temperatures led to synchronized DNA synthesis. The dna A expression under synchronized DNA replication conditions continued to increase during the replication period, but decreased thereafter reflecting autoregulation. The dna Acos mutants at 30°C were elongated suggesting that they may possibly be blocked during the cell division. The DnaA115 protein is defective in its ability to interact with ATP at 30°C, but not at 37°C. Our results suggest that the optimal cell cycle progression and replication initiation in M. tuberculosis requires that the dna A promoter remains active during the replication period and that the DnaA protein is able to interact with ATP." @default.
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- W2000754643 date "2009-01-01" @default.
- W2000754643 modified "2023-09-30" @default.
- W2000754643 title "Synchronous replication initiation in novel<i>Mycobacterium tuberculosis dna</i>A cold-sensitive mutants" @default.
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- W2000754643 doi "https://doi.org/10.1111/j.1365-2958.2008.06523.x" @default.
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