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- W2000756150 abstract "We have cloned a region of 124 kb of the human immunoglobulin lambda light chain locus on chromosome 22 encompassing seven V lambda and seven J-C lambda gene segments. No further C lambda gene segment was found in a region of 35 kb downstream of C lambda 7, which encodes the Ke+Oz- isotype. The C lambda proximal V lambda gene segment V lambda III. 1 is located 14.5 kb upstream of C lambda 1. The five sequenced V lambda genes have the same transcriptional orientation as the J-C lambda gene segments which is likely to be true for the majority of the V lambda gene segments in the human lambda locus and which suggests a deletion mechanism for DNA rearrangement. This is supported by hybridization of V lambda gene probes to germ-line and rearranged DNA from lambda light chain-producing cell lines. Sequences of 23 cDNA clones allow to establish a V lambda subgroup classification based on nucleic acid sequence data and an estimate of the J-C lambda usage." @default.
- W2000756150 created "2016-06-24" @default.
- W2000756150 creator A5057541773 @default.
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- W2000756150 date "1991-06-01" @default.
- W2000756150 modified "2023-09-23" @default.
- W2000756150 title "Vλ and Jλ-Cλ gene segments of the human immunoglobulin λ light chain locus are separated by 14 kb and rearrange by a deletion mechanism" @default.
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- W2000756150 doi "https://doi.org/10.1002/eji.1830210627" @default.
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- W2000756150 hasPublicationYear "1991" @default.
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