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• W2000756851 abstract "A strategy of ex situ electrochemical method has been proposed for investigating the chloride effect on hemoglobin (Hb). Unlike the common electrochemical method that measures the chloride effect on Hb in bulk solution (in situ), the effects of chloride anion on Hb were investigated ex situ by adsorptive transfer voltammetry (AdTV) in this work. Gold electrode modified by self-assembled monolayer of 3-mercaptopropanoic acid (AuE/MPA) was prepared and then incubated in a series of Hb solutions containing different concentrations of chloride anion for adsorbing Hb-Cl (AuE/MPA/Hb-Cl). The resulting electrode was then measured in phosphate buffer solution by cyclic voltammetry. The corresponding voltammograms showed obvious promotion of the direct electron transfer of Hb with remarkable increase of peak currents, decrease of peak-to-peak separations, and negative shift of the formal potentials. As complementation, the adsorption behavior of Hb-Cl on AuE/MPA, the structural information of Hb-Cl, and the electrocatalytic ability of AuE/MPA/Hb-Cl toward hydrogen peroxide were investigated by surface plasmon resonance, circular dichroism spectrum, ultraviolet-visible spectrum and amperometry, respectively. The results indicate that the chloride effect resulted in more electroactive sites of Hb on the surface of electrode. Meanwhile, the specific and nonspecific interactions between Hb and chloride anion can be discriminated from the electrochemical parameters obtained by AdTV." @default.
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• W2000756851 date "2010-04-01" @default.
• W2000756851 modified "2023-10-18" @default.
• W2000756851 title "Investigation of the chloride effect on hemoglobin by adsorptive transfer voltammetry" @default.
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• W2000756851 doi "https://doi.org/10.1016/j.ab.2009.10.049" @default.
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