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- W2000764492 abstract "We constructed a strain of Escherichia coli overproducing 6His-tagged Eco29kI by placing the coding sequence under control of a strong bacteriophage T5 promoter. The yield of 6His-Eco29kI restriction endonuclease expression could be increased to about 20% of the total cellular protein, but inclusion bodies formed consisting of insoluble 6His-Eco29kI protein. We developed a fast and effective protocol for purification of the homogeneous enzyme from both soluble and insoluble fractions and established their identity by catalytic activity assay. The isolated enzymes were tested for recognition specificity and optimal reaction conditions as a function of NaCl and KCl concentrations, temperature, and pH compared with the native Eco29kI restriction endonuclease. The 6His-tagged enzyme retained the specificity of the native protein but had an altered optimum of its catalytic reaction." @default.
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- W2000764492 date "2003-07-01" @default.
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- W2000764492 title "Construction of an overproducing strain, purification, and biochemical characterization of the 6His-Eco29kI restriction endonuclease" @default.
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- W2000764492 doi "https://doi.org/10.1016/s1046-5928(03)00072-x" @default.
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