FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000767321> ?p ?o ?g. }

• W2000767321 endingPage "351" @default.
• W2000767321 startingPage "343" @default.
• W2000767321 abstract "Large unilamellar vesicles (LUV) that contained a fluorescent analog of phosphatidylserine (NBD-PS) were used in model systems to determine the feasibility of employing phosphatidylserine decarboxylase (PS-decarboxylase) to generate asymmetric vesicles and to determine the transbilayer distribution of PS. PS-decarboxylase prepared by sonication of Escherichia coli JA 200 pLC 8-47 was found to be stable in detergent-free buffers and catalyzed the conversion of NBD-PS to NBD-phosphatidylethanolamine (NBD-PE). PS-decarboxylase was capable of decarboxylating virtually all of the NBD-PS present in the outer leaflet of LUV containing a symmetric or asymmetric (outside only) distribution of NBD-PS, but not NBD-PS present in the inner leaflet of the vesicles. The ability of PS-decarboxylase to decarboxylate only NBD-PS located in the outer leaflet of the vesicles was independently verified by resonance energy transfer (between NBD-PS and (lissamine) rhodamine B-labeled phosphatidylethanolamine) and by derivatization with trinitrobenzenesulfonic acid (TNBS). These techniques revealed that the exchangeable pool (the fraction of NBD-PS on the outer leaflet) and the respective fraction of Tnp-(NBD-PS) formed were equivalent to the extent of PS-decarboxylase-mediated decarboxylation of NBD-PS to NBD-PE. These results show that PS-decarboxylase can be used to generate asymmetric vesicles (i.e., PS inside, PE outside) and determine the intrabilayer distribution of PS in model membranes." @default.
• W2000767321 created "2016-06-24" @default.
• W2000767321 creator A5024976931 @default.
• W2000767321 creator A5058765383 @default.
• W2000767321 date "1986-11-01" @default.
• W2000767321 modified "2023-09-26" @default.
• W2000767321 title "Phosphatidylserine decarboxylase: generation of asymmetric vesicles and determination of the transbilayer distribution of fluorescent phosphatidylserine in model membrane systems" @default.
• W2000767321 cites W1491471718 @default.
• W2000767321 cites W1544898320 @default.
• W2000767321 cites W1635858559 @default.
• W2000767321 cites W1653089865 @default.
• W2000767321 cites W1775749144 @default.
• W2000767321 cites W1868710348 @default.
• W2000767321 cites W1963877827 @default.
• W2000767321 cites W1966749786 @default.
• W2000767321 cites W1973762235 @default.
• W2000767321 cites W1976472149 @default.
• W2000767321 cites W1977986845 @default.
• W2000767321 cites W1979729560 @default.
• W2000767321 cites W1983600681 @default.
• W2000767321 cites W2003331008 @default.
• W2000767321 cites W2014279568 @default.
• W2000767321 cites W2016641591 @default.
• W2000767321 cites W2019184035 @default.
• W2000767321 cites W2021851003 @default.
• W2000767321 cites W2030675599 @default.
• W2000767321 cites W2054584898 @default.
• W2000767321 cites W2059526131 @default.
• W2000767321 cites W2062828619 @default.
• W2000767321 cites W2069573006 @default.
• W2000767321 cites W2071782007 @default.
• W2000767321 cites W2073634074 @default.
• W2000767321 cites W2074013555 @default.
• W2000767321 cites W2074295225 @default.
• W2000767321 cites W2091647374 @default.
• W2000767321 cites W2119105467 @default.
• W2000767321 cites W2144892927 @default.
• W2000767321 cites W2153435210 @default.
• W2000767321 cites W2179142653 @default.
• W2000767321 cites W3209951468 @default.
• W2000767321 cites W56064657 @default.
• W2000767321 doi "https://doi.org/10.1016/0005-2736(86)90237-3" @default.
• W2000767321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3535891" @default.
• W2000767321 hasPublicationYear "1986" @default.
• W2000767321 type Work @default.
• W2000767321 sameAs 2000767321 @default.
• W2000767321 citedByCount "14" @default.
• W2000767321 countsByYear W20007673212013 @default.
• W2000767321 countsByYear W20007673212018 @default.
• W2000767321 countsByYear W20007673212019 @default.
• W2000767321 countsByYear W20007673212021 @default.
• W2000767321 countsByYear W20007673212023 @default.
• W2000767321 crossrefType "journal-article" @default.
• W2000767321 hasAuthorship W2000767321A5024976931 @default.
• W2000767321 hasAuthorship W2000767321A5058765383 @default.
• W2000767321 hasConcept C104317684 @default.
• W2000767321 hasConcept C121332964 @default.
• W2000767321 hasConcept C12554922 @default.
• W2000767321 hasConcept C130316041 @default.
• W2000767321 hasConcept C146587185 @default.
• W2000767321 hasConcept C185592680 @default.
• W2000767321 hasConcept C2776330855 @default.
• W2000767321 hasConcept C2778918659 @default.
• W2000767321 hasConcept C2779637612 @default.
• W2000767321 hasConcept C2781170229 @default.
• W2000767321 hasConcept C41625074 @default.
• W2000767321 hasConcept C547475151 @default.
• W2000767321 hasConcept C55493867 @default.
• W2000767321 hasConcept C62520636 @default.
• W2000767321 hasConcept C71240020 @default.
• W2000767321 hasConcept C86803240 @default.
• W2000767321 hasConcept C91881484 @default.
• W2000767321 hasConceptScore W2000767321C104317684 @default.
• W2000767321 hasConceptScore W2000767321C121332964 @default.
• W2000767321 hasConceptScore W2000767321C12554922 @default.
• W2000767321 hasConceptScore W2000767321C130316041 @default.
• W2000767321 hasConceptScore W2000767321C146587185 @default.
• W2000767321 hasConceptScore W2000767321C185592680 @default.
• W2000767321 hasConceptScore W2000767321C2776330855 @default.
• W2000767321 hasConceptScore W2000767321C2778918659 @default.
• W2000767321 hasConceptScore W2000767321C2779637612 @default.
• W2000767321 hasConceptScore W2000767321C2781170229 @default.
• W2000767321 hasConceptScore W2000767321C41625074 @default.
• W2000767321 hasConceptScore W2000767321C547475151 @default.
• W2000767321 hasConceptScore W2000767321C55493867 @default.
• W2000767321 hasConceptScore W2000767321C62520636 @default.
• W2000767321 hasConceptScore W2000767321C71240020 @default.
• W2000767321 hasConceptScore W2000767321C86803240 @default.
• W2000767321 hasConceptScore W2000767321C91881484 @default.
• W2000767321 hasIssue "2" @default.
• W2000767321 hasLocation W20007673211 @default.
• W2000767321 hasLocation W20007673212 @default.
• W2000767321 hasOpenAccess W2000767321 @default.
• W2000767321 hasPrimaryLocation W20007673211 @default.
• W2000767321 hasRelatedWork W1533813159 @default.
• W2000767321 hasRelatedWork W1992601537 @default.
• W2000767321 hasRelatedWork W1993816515 @default.
• W2000767321 hasRelatedWork W2000767321 @default.

• next