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- W2000771513 abstract "Isolation of steroid-binding proteins has been a formidable task because only trace amounts of these materials are normally present. We have developed a specific method based on attachment of the 21 position of cortisol to an insoluble matrix. The steroid derivative used has structural features designed to produce stability to severe elution conditions and to serum and tissue enzymes. Agarose gel was found to be extremely stable to a variety of organic solvents. CBG was purified 20 to 40 fold at a single step. Albumin was the major contaminant but traces of β and γ globulin were also eluted from the column by cortisol." @default.
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- W2000771513 date "1971-10-01" @default.
- W2000771513 modified "2023-09-23" @default.
- W2000771513 title "A ligand column for the purification of steroid-binding proteins" @default.
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- W2000771513 doi "https://doi.org/10.1016/0039-128x(71)90056-0" @default.
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