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- W2000778218 abstract "1. Rabbit antiserum was raised against purified carbamoyl-phosphate synthase ( (ammonia) from rat liver. 2. The antiserum proved to be specific in a double-diffusion test and reacted in an in situ immunohistochemical test on rat liver proteins fractionated on a sodium dodecyl sulphate polyacrylamide gel only in the region where carbamoyl-phosphate syn synthase (ammonia) migrated. 3. This antiserum was used for setting up a radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) in cetyltrimethylammonium bromide extracts of rat liver. To obtain reproducible results in this assay it was necessary to treat the unlabelled ligand with sodium dodecyl sulphate and dithiothreitol. This treatment led to a large increase in the percentage of labelled ligand displaceable by added unlabelled ligand. 4. Radioimmunochemical determination showed that adult rat liver (3-months old) contains 5.5 mg carbamoyl-phosphate synthase (ammonia) protein per gram wet weight." @default.
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- W2000778218 date "1980-04-17" @default.
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- W2000778218 title "Radioimmunochemical determination of carbamoyl-phosphate synthase (ammonia) content of adult rat liver" @default.
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- W2000778218 doi "https://doi.org/10.1016/0304-4165(80)90262-7" @default.
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