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- W2000778635 abstract "Specific dihydrotestosterone (DHT) binding was demonstrated in the cytosol of hamster sebaceous glands. When the cytosol was incubated with [3H]-DHT and subjected to sucrose gradient centrifugation, a sharp peak of radioactivity was observed in the 7–8S region. This binding showed a high affinity for DHT (KD = 1.5 × 10−9 M). Testosterone was also bound in that region, if to a much lesser extent. Competition among various kinds of excess cold steroids revealed their binding affinities in the decreasing order, as follows: DHT, cyproterone acetate, testosterone, 17β-estradiol, 5α-andros-tane-3α, 17β-diol, progesterone, androstenedione, cortisol, and androsterone. For the demonstration of intranuclear binding, the homogenate was incubated with [3H]-DHT and the extracts from crude nuclei were filtered through Sephadex G-100. Three peaks of radioactivity were observed. The second peak, which approximated the elution of chymotrypsinogen A, appeared to represent the specific binding to the androgen from the following results: saturated at 7 × 10−9 M of DHT, completely abolished by an excess amount of unlabeled DHT and decreased by more than 75% by 100-fold excess of cyproterone acetate. Incubation with [3H]-testosterone did not result in the formation of the second peak. The formation of this [3H]-DHT-macromolecule complex in the nuclei required cytoplasmic factors." @default.
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- W2000778635 date "1978-02-01" @default.
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- W2000778635 title "Androgen binding to cytosol and nuclei of hamster sebaceous glands" @default.
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- W2000778635 doi "https://doi.org/10.1016/0022-4731(78)90082-1" @default.
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