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- W2000779903 abstract "To demonstrate the superoxide radical (·O2−) -scavenging activity of 2-mercaptoethylamine (MEA), we investigated the induction of the oxidative stress-inducible(soi) gene fused lacZ gene (soi-28:: lacZ) by the use of paraquat as a source of ·O2−. When MEA or cysteine was added to the cultures before paraquat treatment, soi gene induction by paraquat was significantly inhibited. However, a high quantity of ascorbic acid (5 mm) inhibited soi gene induction by paraquat far less than MEA or cysteine did. The induction of soi gene induction by MEA exhibited a dose-dependent manner in the range of over 0.2 mm. The antagonistic molecules on the radioprotective action of MEA, ascorbic acid and cysteine did not counteract MEA action on the inhibition of paraquat-mediated soi gene induction. To clarify that the MEA action on the inhibition of paraquat-mediated soi gene induction may be due, in part, to ·O2−-scavenging activity, we investigated the ability of MEA to inhibit the nitroblue tetrazolium (NBT) reduction mediated by ·O2−generated in the xanthine oxidase/hypoxanthine system in vitro. At concentrations above 1 mm, MEA effectively inhibited the NBT reduction in a concentration-dependent fashion. Our results demonstrated that MEA has an ability to scavenge·O2− , and so protects against ·O2−-mediated damage." @default.
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- W2000779903 date "2000-11-01" @default.
- W2000779903 modified "2023-09-27" @default.
- W2000779903 title "2-Mercaptoethylamine, Radioprotector, inhibits the induction of the oxidative stress-inducible (soi) gene by paraquat in Escherichia coli" @default.
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- W2000779903 doi "https://doi.org/10.1006/phrs.2000.0708" @default.
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