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- W2000783535 abstract "Sensory and sympathetic nerve cells were dissociated by trypsinization and cultured in CO2-conditioned liquid media. Eagle's medium alone and Eagle's medium supplemented with 10% horse serum proved to be inadequate for the survival and maintenance of these cells. Addition of a specific protein factor (nerve growth factor) at a concentration of 0.05 μg per milliliter of the culture medium, resulted in the survival and maintenance of the sensory and sympathetic nerve cells. These results add new evidence in support of the concept that the NGF plays an essential role in the life of the above cells." @default.
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- W2000783535 modified "2023-10-11" @default.
- W2000783535 title "Essential role of the nerve growth factor in the survival and maintenance of dissociated sensory and sympathetic embryonic nerve cells in vitro" @default.
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- W2000783535 doi "https://doi.org/10.1016/0012-1606(63)90149-0" @default.
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