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- W2000783650 abstract "Protein synthesis in wool follicles was studied by incubating plucked wool fibers at 37° C in Krebs-Ringer-phosphate buffer containing 14 C-leucine and glucose. The incorporation of 14 C-leucine by the plucked fibers was maintained for 12-24 hours in the presence of glucose. Autoradiographs showed that the incorporated radioactivity was mainly confined to the bulb region of the fiber. Initially most of the 14 C was present in wool root proteins which were dissolved or dispersed by 8M urea. During incubation these proteins became increasingly insoluble in 8M urea. After 24 hours' incubation the wool roots appeared completely urea-resistant and almost all of the incorporated 14 C was present in urea-insoluble material. This change in solubility was probably due to non-enzymatic oxidation of thiol groups and was catalyzed by cupric ions in the presence of atmospheric oxygen. Electrophoresis of the wool root proteins synthesized in vitro showed that 14 C-leucine was mainly incorporated into low-sulfur keratin proteins. Despite the apparent difficulties in maintaining the urea-solubility of the wool roots in vitro , this synthesis of low-sulfur protein in the bulb region of the plucked fibers indicates that keratin biosynthesis can be satisfactorily studied in vitro by this method." @default.
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- W2000783650 date "1971-11-01" @default.
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- W2000783650 title "The Incorporation of 14C-Leucine into the Proteins of Plucked Wool Follicles" @default.
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- W2000783650 doi "https://doi.org/10.1111/1523-1747.ep12292340" @default.
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