FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000811971> ?p ?o ?g. }

• W2000811971 endingPage "375" @default.
• W2000811971 startingPage "361" @default.
• W2000811971 abstract "Apolipoprotein E (apoE), an antiatherogenic apolipoprotein, plays a significant role in the metabolism of lipoproteins. It lowers plasma lipid levels by acting as a ligand for the low-density lipoprotein receptor (LDLr) family of proteins, in addition to playing a role in promoting macrophage cholesterol efflux in atherosclerotic lesions. The objective of this study is to examine the effect of acrolein modification on the structure and function of rat apoE and to determine the sites and nature of modification by mass spectrometry. Acrolein is a highly reactive aldehyde, which is generated endogenously as one of the products of lipid peroxidation and is present in the environment in pollutants such as tobacco smoke and heated oils. In initial studies, acrolein-modified apoE was identified by immunoprecipitation using an acrolein-lysine specific antibody in the plasma of 10-week old male rats that were exposed to filtered air (FA) or low doses of environmental tobacco smoke (ETS). While both groups displayed acrolein-modified apoE in the lipoprotein fraction, the ETS group had higher levels in the lipid-free fraction compared with the FA group. This observation provided the rationale to further investigate the effect of acrolein modification on rat apoE at a molecular level. Treatment of recombinant rat apoE with a 10-fold molar excess of acrolein resulted in (i) a significant decrease in lipid-binding and cholesterol efflux abilities, (ii) impairment in the LDLr- and heparin-binding capabilities, and (iii) significant alterations in the overall stability of the protein. The disruption in the functional abilities is attributed directly or indirectly to acrolein modification yielding an aldimine adduct at K149 and K155 (+38); a propanal adduct at K135 and K138 (+56); an Nε-(3-methylpyridinium)lysine (MP-lysine) at K64, K67, and K254 (+76), and an Nε-(3-formyl-3,4-dehydropiperidino)lysine (FDP-lysine) derivative at position K68 (+94), as determined by matrix-assisted laser desorption/ionization–time of flight/time of flight mass spectrometry (MALDI-TOF/TOF MS). The loss of function may also be attributed to alterations in the overall fold of the protein as noted by changes in the guanidine HCl-induced unfolding pattern and to protein cross-linking. Overall, disruption of the structural and functional integrity of apoE by oxidative modification of essential lysine residues by acrolein is expected to affect its role in maintaining plasma cholesterol homeostasis and lead to dysregulation in lipid metabolism." @default.
• W2000811971 created "2016-06-24" @default.
• W2000811971 creator A5007935241 @default.
• W2000811971 creator A5011722417 @default.
• W2000811971 creator A5014027181 @default.
• W2000811971 creator A5014950932 @default.
• W2000811971 creator A5027437494 @default.
• W2000811971 creator A5041660090 @default.
• W2000811971 creator A5042511606 @default.
• W2000811971 creator A5043009880 @default.
• W2000811971 creator A5055822025 @default.
• W2000811971 creator A5078821297 @default.
• W2000811971 date "2014-01-08" @default.
• W2000811971 modified "2023-10-10" @default.
• W2000811971 title "Acrolein Modification Impairs Key Functional Features of Rat Apolipoprotein E: Identification of Modified Sites by Mass Spectrometry" @default.
• W2000811971 cites W1475896266 @default.
• W2000811971 cites W1487164341 @default.
• W2000811971 cites W1505230657 @default.
• W2000811971 cites W1529259401 @default.
• W2000811971 cites W1553780630 @default.
• W2000811971 cites W1559121591 @default.
• W2000811971 cites W1900087291 @default.
• W2000811971 cites W1931341062 @default.
• W2000811971 cites W1964404215 @default.
• W2000811971 cites W1966945429 @default.
• W2000811971 cites W1968347471 @default.
• W2000811971 cites W1969321910 @default.
• W2000811971 cites W1973065853 @default.
• W2000811971 cites W1980352486 @default.
• W2000811971 cites W1983577234 @default.
• W2000811971 cites W1986281316 @default.
• W2000811971 cites W1986439388 @default.
• W2000811971 cites W1994543234 @default.
• W2000811971 cites W1998052006 @default.
• W2000811971 cites W2000913322 @default.
• W2000811971 cites W2006599070 @default.
• W2000811971 cites W2006992003 @default.
• W2000811971 cites W2007819827 @default.
• W2000811971 cites W2011511504 @default.
• W2000811971 cites W2017319919 @default.
• W2000811971 cites W2019299857 @default.
• W2000811971 cites W2020888421 @default.
• W2000811971 cites W2021452361 @default.
• W2000811971 cites W2029070177 @default.
• W2000811971 cites W2030720809 @default.
• W2000811971 cites W2035252462 @default.
• W2000811971 cites W2037019145 @default.
• W2000811971 cites W2037813889 @default.
• W2000811971 cites W2049660003 @default.
• W2000811971 cites W2050776161 @default.
• W2000811971 cites W2051394059 @default.
• W2000811971 cites W2053669884 @default.
• W2000811971 cites W2053826548 @default.
• W2000811971 cites W2056215168 @default.
• W2000811971 cites W2057522312 @default.
• W2000811971 cites W2063055336 @default.
• W2000811971 cites W2063059534 @default.
• W2000811971 cites W2064450689 @default.
• W2000811971 cites W2064992681 @default.
• W2000811971 cites W2066531899 @default.
• W2000811971 cites W2067160010 @default.
• W2000811971 cites W2070984401 @default.
• W2000811971 cites W2078442135 @default.
• W2000811971 cites W2087151602 @default.
• W2000811971 cites W2088400951 @default.
• W2000811971 cites W2090515271 @default.
• W2000811971 cites W2090688839 @default.
• W2000811971 cites W2090843520 @default.
• W2000811971 cites W2093648180 @default.
• W2000811971 cites W2101101564 @default.
• W2000811971 cites W2101105961 @default.
• W2000811971 cites W2104215271 @default.
• W2000811971 cites W2106771721 @default.
• W2000811971 cites W2107887632 @default.
• W2000811971 cites W2115220026 @default.
• W2000811971 cites W2121244624 @default.
• W2000811971 cites W2122062568 @default.
• W2000811971 cites W2123347238 @default.
• W2000811971 cites W2124987022 @default.
• W2000811971 cites W2127908806 @default.
• W2000811971 cites W2129883875 @default.
• W2000811971 cites W2137511741 @default.
• W2000811971 cites W2139266365 @default.
• W2000811971 cites W2142403172 @default.
• W2000811971 cites W2150436995 @default.
• W2000811971 cites W2153609395 @default.
• W2000811971 cites W2154521687 @default.
• W2000811971 cites W2162458714 @default.
• W2000811971 cites W2164280326 @default.
• W2000811971 cites W2165320750 @default.
• W2000811971 cites W2184987640 @default.
• W2000811971 cites W2189057339 @default.
• W2000811971 cites W2316107067 @default.
• W2000811971 cites W2317841552 @default.
• W2000811971 cites W2326202471 @default.
• W2000811971 cites W2328211663 @default.
• W2000811971 cites W98152484 @default.
• W2000811971 doi "https://doi.org/10.1021/bi401404u" @default.

• next