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• W2000812320 endingPage "694" @default.
• W2000812320 startingPage "687" @default.
• W2000812320 abstract "Here, the sequence in the hprt gene of the duplication mutant SPD8 originating from V79 Chinese hamster cells was determined. The duplication arose after non-homologous recombination between exon 6 and intron 7, resulting in an extra copy of the 3′ portion of exon 6, of exon 7 and of flanking intron regions. Only a duplication of exon 7 is present in the mRNA, since the duplicated exon 6 lacks its 5′ splice site and is removed during RNA processing. The findings in this study suggest that the non-homologous recombination mechanism which occurred here may have been initiated by endonucleases, rather than by a spontaneous double strand break. Subsequently, 14 spontaneous SPD8 revertants with a functional hprt gene were isolated and characterized using PCR and sequencing. The data revealed that although the SPD8 cell line arose by non-homologous recombination, it reverts spontaneously by homologous recombination. Interestingly, the downstream copy of exon 7 was restored by this process. This was indicated by the presence of a specific mutation, a T-to-G transversion, close to the breakpoint, a characteristic unique to the SPD8 clone. Our results suggest that the spontaneous reversion of this cell line by homologous recombination may involve an exchange, rather than a conversion mechanism." @default.
• W2000812320 created "2016-06-24" @default.
• W2000812320 creator A5007181246 @default.
• W2000812320 creator A5029296072 @default.
• W2000812320 creator A5073400533 @default.
• W2000812320 date "1998-06-01" @default.
• W2000812320 modified "2023-10-18" @default.
• W2000812320 title "A partial HPRT gene duplication generated by non-homologous recombination in V79 chinese hamster cells is eliminated by homologous recombination" @default.
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• W2000812320 doi "https://doi.org/10.1006/jmbi.1998.1809" @default.
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• W2000812320 hasPublicationYear "1998" @default.
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