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- W2000818612 abstract "The transformation of potato (Solanum tuberosum L. cv. DĂŠsirĂŠe) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm-3." @default.
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- W2000818612 date "2010-03-01" @default.
- W2000818612 modified "2023-10-03" @default.
- W2000818612 title "Transformation of potato via Agrobacterium coated microparticle bombardment" @default.
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- W2000818612 doi "https://doi.org/10.1007/s10535-010-0022-2" @default.
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