FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000821594> ?p ?o ?g. }

• W2000821594 endingPage "133" @default.
• W2000821594 startingPage "127" @default.
• W2000821594 abstract "Oligonucleotides containing a photocleavable biotin (5′-PC-biotin) were analyzed by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) with wavelengths in the ultraviolet (UV) and infrared (IR) from solution and after capture on streptavidin-coated agarose or magnetic beads. The analysis was used to monitor the release of the oligonucleotides as a result of photochemical cleavage of the biotinylated linker. Near-UV pulses (UV-MALDI) led to predominant release of the photocleaved product. In contrast, only the uncleaved analyte was detected using IR pulses (IR-MALDI). Results from MALDI analysis are also presented for DNA containing a photocleavable 5′-amino group which can be covalently linked to a variety of activated surfaces and marker molecules. In a demonstration of this approach, a 5′-PC-biotinylated 49 nt RNA oligonucleotide was enzymatically synthesized using a PC-biotin-r(AG) dinucleotide primer, captured on streptavidin coated magnetic beads and analyzed by UV-MALDI. Potential applications of photocleavable linkers combined with MALDI for the analysis of nucleic acids are discussed." @default.
• W2000821594 created "2016-06-24" @default.
• W2000821594 creator A5009458005 @default.
• W2000821594 creator A5014887955 @default.
• W2000821594 creator A5024449091 @default.
• W2000821594 creator A5038989241 @default.
• W2000821594 creator A5091207409 @default.
• W2000821594 creator A5091590585 @default.
• W2000821594 date "1999-12-01" @default.
• W2000821594 modified "2023-10-18" @default.
• W2000821594 title "Matrix-assisted laser desorption/ionization mass spectrometry of DNA using photocleavable biotin" @default.
• W2000821594 cites W1965229422 @default.
• W2000821594 cites W1967481754 @default.
• W2000821594 cites W1978349009 @default.
• W2000821594 cites W1991602715 @default.
• W2000821594 cites W1993176979 @default.
• W2000821594 cites W2012221528 @default.
• W2000821594 cites W2021852038 @default.
• W2000821594 cites W2022733717 @default.
• W2000821594 cites W2022850426 @default.
• W2000821594 cites W2040033652 @default.
• W2000821594 cites W2053858231 @default.
• W2000821594 cites W2060935559 @default.
• W2000821594 cites W2069746331 @default.
• W2000821594 cites W2070825600 @default.
• W2000821594 cites W2077309813 @default.
• W2000821594 cites W2089611548 @default.
• W2000821594 cites W2093381385 @default.
• W2000821594 cites W2117098825 @default.
• W2000821594 cites W2118889049 @default.
• W2000821594 cites W2156922318 @default.
• W2000821594 cites W4251700885 @default.
• W2000821594 doi "https://doi.org/10.1016/s1050-3862(99)00049-2" @default.
• W2000821594 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10796995" @default.
• W2000821594 hasPublicationYear "1999" @default.
• W2000821594 type Work @default.
• W2000821594 sameAs 2000821594 @default.
• W2000821594 citedByCount "11" @default.
• W2000821594 countsByYear W20008215942012 @default.
• W2000821594 countsByYear W20008215942013 @default.
• W2000821594 crossrefType "journal-article" @default.
• W2000821594 hasAuthorship W2000821594A5009458005 @default.
• W2000821594 hasAuthorship W2000821594A5014887955 @default.
• W2000821594 hasAuthorship W2000821594A5024449091 @default.
• W2000821594 hasAuthorship W2000821594A5038989241 @default.
• W2000821594 hasAuthorship W2000821594A5091207409 @default.
• W2000821594 hasAuthorship W2000821594A5091590585 @default.
• W2000821594 hasBestOaLocation W20008215941 @default.
• W2000821594 hasConcept C10390740 @default.
• W2000821594 hasConcept C129312508 @default.
• W2000821594 hasConcept C150394285 @default.
• W2000821594 hasConcept C162356407 @default.
• W2000821594 hasConcept C162711632 @default.
• W2000821594 hasConcept C178790620 @default.
• W2000821594 hasConcept C185592680 @default.
• W2000821594 hasConcept C19014433 @default.
• W2000821594 hasConcept C204628709 @default.
• W2000821594 hasConcept C21951064 @default.
• W2000821594 hasConcept C2776190903 @default.
• W2000821594 hasConcept C2778204606 @default.
• W2000821594 hasConcept C2778476535 @default.
• W2000821594 hasConcept C43617362 @default.
• W2000821594 hasConcept C552990157 @default.
• W2000821594 hasConcept C55493867 @default.
• W2000821594 hasConcept C75280812 @default.
• W2000821594 hasConceptScore W2000821594C10390740 @default.
• W2000821594 hasConceptScore W2000821594C129312508 @default.
• W2000821594 hasConceptScore W2000821594C150394285 @default.
• W2000821594 hasConceptScore W2000821594C162356407 @default.
• W2000821594 hasConceptScore W2000821594C162711632 @default.
• W2000821594 hasConceptScore W2000821594C178790620 @default.
• W2000821594 hasConceptScore W2000821594C185592680 @default.
• W2000821594 hasConceptScore W2000821594C19014433 @default.
• W2000821594 hasConceptScore W2000821594C204628709 @default.
• W2000821594 hasConceptScore W2000821594C21951064 @default.
• W2000821594 hasConceptScore W2000821594C2776190903 @default.
• W2000821594 hasConceptScore W2000821594C2778204606 @default.
• W2000821594 hasConceptScore W2000821594C2778476535 @default.
• W2000821594 hasConceptScore W2000821594C43617362 @default.
• W2000821594 hasConceptScore W2000821594C552990157 @default.
• W2000821594 hasConceptScore W2000821594C55493867 @default.
• W2000821594 hasConceptScore W2000821594C75280812 @default.
• W2000821594 hasIssue "1-4" @default.
• W2000821594 hasLocation W20008215941 @default.
• W2000821594 hasLocation W20008215942 @default.
• W2000821594 hasOpenAccess W2000821594 @default.
• W2000821594 hasPrimaryLocation W20008215941 @default.
• W2000821594 hasRelatedWork W1989697812 @default.
• W2000821594 hasRelatedWork W2000821594 @default.
• W2000821594 hasRelatedWork W2004680470 @default.
• W2000821594 hasRelatedWork W2007388934 @default.
• W2000821594 hasRelatedWork W2051488870 @default.
• W2000821594 hasRelatedWork W2093386627 @default.
• W2000821594 hasRelatedWork W2147506087 @default.
• W2000821594 hasRelatedWork W2164375416 @default.
• W2000821594 hasRelatedWork W3034041648 @default.
• W2000821594 hasRelatedWork W3135136565 @default.
• W2000821594 hasVolume "16" @default.

• next