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• W2000821845 abstract "Inhibition of kynurenine aminotransferases (KATs) is a strategy to therapeutically reduce levels of kynurenic acid (KYNA), an endogenous antagonist of glutamatergic N-methyl-d-aspartate (NMDA) and cholinergic α7 nicotinic receptors. Several methods of measuring KAT activity in vitro have been developed, but none is well-suited to high throughput and automation. In this article, we describe a modification of existing high-performance liquid chromatography (HPLC)-based methods that enables the development of a 96-well microplate assay in both enzyme- and cell-based formats using human KAT I as an example. KYNA enzymatically produced from l-kynurenine is measured directly in a reaction mixture fluorimetrically." @default.
• W2000821845 created "2016-06-24" @default.
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• W2000821845 date "2011-02-01" @default.
• W2000821845 modified "2023-09-24" @default.
• W2000821845 title "Development of a microplate fluorescence assay for kynurenine aminotransferase" @default.
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• W2000821845 doi "https://doi.org/10.1016/j.ab.2010.10.037" @default.
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