Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000827897> ?p ?o ?g. }
- W2000827897 endingPage "2700" @default.
- W2000827897 startingPage "2690" @default.
- W2000827897 abstract "Summary Complex regional pain syndrome patient sera contain functional active autoantibodies against the β2 adrenergic receptor and muscarinic-2 receptor, supporting the concept of an autoimmune etiology in CRPS. Complex regional pain syndrome (CRPS) is a painful condition affecting one or more extremities of the body, marked by a wide variety of symptoms and signs that are often difficult to manage because the pathophysiology is incompletely understood. Thus, diverse treatments might be ineffective. A recent report revealed the presence of autoantibodies against differentiated autonomic neurons in CRPS patients. However, it remained unclear how the antibodies act in the development of CRPS. We therefore aimed to characterize these antibodies and identify target antigens. Functional properties of affinity-purified immunoglobulin G of control subjects or CRPS patients were assessed using a cardiomyocyte bioassay. Putative corresponding receptors were identified using antagonistic drugs, and synthesized peptide sequences corresponding to segments of these receptors were used to identify the target epitopes. Chinese hamster ovary cells were transfected with putative receptors to ensure observed binding. Further, changes in the intracellular Ca2+ concentration induced by agonistic immunoglobulin G were measured using the Ca2+-sensitive fluorescent dye fura-2 assay. Herein, we demonstrate the presence of autoantibodies in a subset of CRPS patients with agonistic-like properties on the β2 adrenergic receptor and/or the muscarinic-2 receptor. We identified these autoantibodies as immunoglobulin G directed against peptide sequences from the second extracellular loop of these receptors. The identification of functionally active autoantibodies in serum samples from CRPS patients supports an autoimmune pathogenesis of CRPS. Thus, our findings contribute to the further understanding of this disease, could help in the diagnosis in future, and encourage new treatment strategies focusing on the immune system." @default.
- W2000827897 created "2016-06-24" @default.
- W2000827897 creator A5022343895 @default.
- W2000827897 creator A5026211760 @default.
- W2000827897 creator A5031592308 @default.
- W2000827897 creator A5037021702 @default.
- W2000827897 creator A5038514497 @default.
- W2000827897 creator A5060330435 @default.
- W2000827897 creator A5062939642 @default.
- W2000827897 creator A5073046515 @default.
- W2000827897 creator A5075282190 @default.
- W2000827897 creator A5079394257 @default.
- W2000827897 creator A5084769052 @default.
- W2000827897 creator A5085784398 @default.
- W2000827897 date "2011-12-01" @default.
- W2000827897 modified "2023-10-17" @default.
- W2000827897 title "Autoimmunity against the β2 adrenergic receptor and muscarinic-2 receptor in complex regional pain syndrome" @default.
- W2000827897 cites W1504180470 @default.
- W2000827897 cites W1583258274 @default.
- W2000827897 cites W1765396147 @default.
- W2000827897 cites W1964105353 @default.
- W2000827897 cites W1968412767 @default.
- W2000827897 cites W1973439033 @default.
- W2000827897 cites W1975260965 @default.
- W2000827897 cites W1976024859 @default.
- W2000827897 cites W1976213628 @default.
- W2000827897 cites W1982038074 @default.
- W2000827897 cites W1982278129 @default.
- W2000827897 cites W1985016377 @default.
- W2000827897 cites W1985734972 @default.
- W2000827897 cites W1987936028 @default.
- W2000827897 cites W1995166330 @default.
- W2000827897 cites W1996323606 @default.
- W2000827897 cites W1997388908 @default.
- W2000827897 cites W1998252102 @default.
- W2000827897 cites W1999950549 @default.
- W2000827897 cites W2003663903 @default.
- W2000827897 cites W2009265781 @default.
- W2000827897 cites W2012701523 @default.
- W2000827897 cites W2014571855 @default.
- W2000827897 cites W2032273455 @default.
- W2000827897 cites W2034211901 @default.
- W2000827897 cites W2043607792 @default.
- W2000827897 cites W2045120834 @default.
- W2000827897 cites W2046794040 @default.
- W2000827897 cites W2048863213 @default.
- W2000827897 cites W2049894288 @default.
- W2000827897 cites W2058652462 @default.
- W2000827897 cites W2063882570 @default.
- W2000827897 cites W2065227289 @default.
- W2000827897 cites W2069356348 @default.
- W2000827897 cites W2072851012 @default.
- W2000827897 cites W2074747030 @default.
- W2000827897 cites W2077232948 @default.
- W2000827897 cites W2080683650 @default.
- W2000827897 cites W2093169891 @default.
- W2000827897 cites W2096098509 @default.
- W2000827897 cites W2096328516 @default.
- W2000827897 cites W2098167550 @default.
- W2000827897 cites W2102979693 @default.
- W2000827897 cites W2113332244 @default.
- W2000827897 cites W2119694615 @default.
- W2000827897 cites W2123128921 @default.
- W2000827897 cites W2140690831 @default.
- W2000827897 cites W2147599802 @default.
- W2000827897 cites W2151771466 @default.
- W2000827897 cites W2152409713 @default.
- W2000827897 cites W2158396577 @default.
- W2000827897 cites W2164791359 @default.
- W2000827897 cites W2312672018 @default.
- W2000827897 cites W4240341044 @default.
- W2000827897 doi "https://doi.org/10.1016/j.pain.2011.06.012" @default.
- W2000827897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21816540" @default.
- W2000827897 hasPublicationYear "2011" @default.
- W2000827897 type Work @default.
- W2000827897 sameAs 2000827897 @default.
- W2000827897 citedByCount "165" @default.
- W2000827897 countsByYear W20008278972012 @default.
- W2000827897 countsByYear W20008278972013 @default.
- W2000827897 countsByYear W20008278972014 @default.
- W2000827897 countsByYear W20008278972015 @default.
- W2000827897 countsByYear W20008278972016 @default.
- W2000827897 countsByYear W20008278972017 @default.
- W2000827897 countsByYear W20008278972018 @default.
- W2000827897 countsByYear W20008278972019 @default.
- W2000827897 countsByYear W20008278972020 @default.
- W2000827897 countsByYear W20008278972021 @default.
- W2000827897 countsByYear W20008278972022 @default.
- W2000827897 countsByYear W20008278972023 @default.
- W2000827897 crossrefType "journal-article" @default.
- W2000827897 hasAuthorship W2000827897A5022343895 @default.
- W2000827897 hasAuthorship W2000827897A5026211760 @default.
- W2000827897 hasAuthorship W2000827897A5031592308 @default.
- W2000827897 hasAuthorship W2000827897A5037021702 @default.
- W2000827897 hasAuthorship W2000827897A5038514497 @default.
- W2000827897 hasAuthorship W2000827897A5060330435 @default.
- W2000827897 hasAuthorship W2000827897A5062939642 @default.
- W2000827897 hasAuthorship W2000827897A5073046515 @default.