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- W2000828010 abstract "Abstract An enzyme that catalyses the transfer of a glucose unit from UDPG to limonoids was isolated from the albedo tissue of pummelo fruit ( Citrus grandis Osbeck) in electrophoretically homogeneous form. The enzyme was purified to 180-fold by a combination of (NH 4 ) 2 SO 4 fractionation, ion exchange chromatography on DEAE-cellulose and DEAE-Toyopearl. SDS-PAGE showed a molecular weight of 55 kDa for the enzyme. The purified enzyme, limonoid glucosyltransferase, displayed an optimum activity at pH 7.8 and 37 °C with apparent K m values of 65 and 200 μM for limonin and UDPG, respectively. Mn 2+ and Co 2+ stimulated the enzyme activity by 33 and 30%, respectively, while EDTA completely inhibited it. Cu 2+ , Hg 2+ and diethyl pyrocarbonate also inhibited the enzyme, indicating a possible role of histidine in catalysis. The enzyme was stable at 4 °C for 6 months in Tris–HCl buffer, pH 7.5." @default.
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- W2000828010 date "2002-04-01" @default.
- W2000828010 modified "2023-09-26" @default.
- W2000828010 title "Isolation and characterization of limonoid glucosyltransferase from pummelo albedo tissue" @default.
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- W2000828010 doi "https://doi.org/10.1016/s0308-8146(01)00300-4" @default.
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