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• W2000829013 abstract "To facilitate searching for genes encoding cell membrane proteins, we developed a method for isolating cDNAs that contain sequences for hydrophobic transmembrane runs. This cloning strategy, termed the “transmembrane (TM) trap method,” utilizes a vector that directs the cell surface expression of mouse CD4 fusion protein when an insert encoding hydrophobic transmembrane sequences is cloned in-frame with correct orientation. We applied this novel method to isolation of cytokine receptor cDNAs. Our strategy enabled efficient isolation of relatively rare species encoding receptors such as IL-2Rγ, IL-3Rβ, IL-4Rα, IL-5Rα, and IL-6Rα. This method also could be used to isolate cDNAs for intracellular molecules with a transmembrane region, e.g., bcl-2. These results indicate that the TM trap method provides an efficient cloning strategy for identification of various families of genes encoding proteins with one or more transmembrane regions." @default.
• W2000829013 created "2016-06-24" @default.
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• W2000829013 date "2001-12-01" @default.
• W2000829013 modified "2023-09-29" @default.
• W2000829013 title "A Transmembrane Trap Method for Efficient Cloning of Genes Encoding Proteins Possessing Transmembrane Domain" @default.
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• W2000829013 doi "https://doi.org/10.1006/bbrc.2001.6097" @default.
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