FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000832493> ?p ?o ?g. }

• W2000832493 endingPage "424" @default.
• W2000832493 startingPage "409" @default.
• W2000832493 abstract "Human neuropeptide Y (hNPY) is a potent vasoconstrictor peptide of 36 aminoacid residues. We isolated hybridomas secreting four monoclonal antibodies directed against various epitopes of neuropeptide Y and studied their cross-reactivity with peptide YY (PYY) and the pancreatic polypeptide (PP), two peptides sharing sequence homologies with hNPY (respectively 70% and 50%). The antibody NPY02 is an IgGl with a Ka of 5.5 x 1010 liters/mol. It binds to the 11-24 region of NPY (IC50 = 2 x 10-7M), does not recognize PP but cross-reacts weakly with PYY. Antibodies NPY03 and NPY05 are IgG2 with respective Ka's of 6.7 x 109 and 2.5 x 1010 liters/mol. They interact with a C-terminal epitope on NPY (NPY 27-34, IC50 = 2 x 10-9 M for NPY03 and NPY 32-36, IC50 = 1 x 10-9 M for NPY05). These two antibodies cross-react with PYY whereas only NPY05 binds PP. NPY05 is unable to bind the free acid form of neuropeptide Y. The 32-36 COOH free subpeptide is recognized 50,000 less efficiently by NPY05 than its amidated form. Antibody NPY04 is an IgG3 with a Ka of 3.8 x 108 liters/mol. It recognizes a N-terminal epitope between aminoacids 1 and 12 (IC50 = 2.5 x 10-6 M). NPY04 interacts weakly with PYY but not detectably with PP. These results obtained with 4 different monoclonal antibodies demonstrate the presence of at least four epitopes on hNPY, two of them being continuous. These antibodies will be used to study the interaction of NPY with its receptor and to develop sensitive and specific assays for determination of NPY concentrations in biological fluids." @default.
• W2000832493 created "2016-06-24" @default.
• W2000832493 creator A5032738255 @default.
• W2000832493 creator A5034612906 @default.
• W2000832493 creator A5036167932 @default.
• W2000832493 creator A5036806632 @default.
• W2000832493 creator A5039143614 @default.
• W2000832493 creator A5040661239 @default.
• W2000832493 creator A5049779938 @default.
• W2000832493 date "1992-08-01" @default.
• W2000832493 modified "2023-10-18" @default.
• W2000832493 title "Production and Characterization of Four Anti-Neuropeptide Y Monoclonal Antibodies" @default.
• W2000832493 cites W2010722974 @default.
• W2000832493 cites W2021014505 @default.
• W2000832493 cites W2070943463 @default.
• W2000832493 cites W2076468414 @default.
• W2000832493 cites W2080580789 @default.
• W2000832493 cites W2125076183 @default.
• W2000832493 cites W2320556745 @default.
• W2000832493 doi "https://doi.org/10.1089/hyb.1992.11.409" @default.
• W2000832493 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1383123" @default.
• W2000832493 hasPublicationYear "1992" @default.
• W2000832493 type Work @default.
• W2000832493 sameAs 2000832493 @default.
• W2000832493 citedByCount "56" @default.
• W2000832493 countsByYear W20008324932012 @default.
• W2000832493 countsByYear W20008324932015 @default.
• W2000832493 countsByYear W20008324932016 @default.
• W2000832493 countsByYear W20008324932019 @default.
• W2000832493 countsByYear W20008324932020 @default.
• W2000832493 countsByYear W20008324932022 @default.
• W2000832493 crossrefType "journal-article" @default.
• W2000832493 hasAuthorship W2000832493A5032738255 @default.
• W2000832493 hasAuthorship W2000832493A5034612906 @default.
• W2000832493 hasAuthorship W2000832493A5036167932 @default.
• W2000832493 hasAuthorship W2000832493A5036806632 @default.
• W2000832493 hasAuthorship W2000832493A5039143614 @default.
• W2000832493 hasAuthorship W2000832493A5040661239 @default.
• W2000832493 hasAuthorship W2000832493A5049779938 @default.
• W2000832493 hasConcept C104317684 @default.
• W2000832493 hasConcept C118303440 @default.
• W2000832493 hasConcept C153911025 @default.
• W2000832493 hasConcept C159654299 @default.
• W2000832493 hasConcept C167625842 @default.
• W2000832493 hasConcept C170493617 @default.
• W2000832493 hasConcept C185592680 @default.
• W2000832493 hasConcept C195616568 @default.
• W2000832493 hasConcept C202751555 @default.
• W2000832493 hasConcept C203014093 @default.
• W2000832493 hasConcept C2777752497 @default.
• W2000832493 hasConcept C2778108375 @default.
• W2000832493 hasConcept C2778563252 @default.
• W2000832493 hasConcept C2779281246 @default.
• W2000832493 hasConcept C2780796126 @default.
• W2000832493 hasConcept C542903549 @default.
• W2000832493 hasConcept C55493867 @default.
• W2000832493 hasConcept C71315377 @default.
• W2000832493 hasConcept C86803240 @default.
• W2000832493 hasConcept C93984277 @default.
• W2000832493 hasConceptScore W2000832493C104317684 @default.
• W2000832493 hasConceptScore W2000832493C118303440 @default.
• W2000832493 hasConceptScore W2000832493C153911025 @default.
• W2000832493 hasConceptScore W2000832493C159654299 @default.
• W2000832493 hasConceptScore W2000832493C167625842 @default.
• W2000832493 hasConceptScore W2000832493C170493617 @default.
• W2000832493 hasConceptScore W2000832493C185592680 @default.
• W2000832493 hasConceptScore W2000832493C195616568 @default.
• W2000832493 hasConceptScore W2000832493C202751555 @default.
• W2000832493 hasConceptScore W2000832493C203014093 @default.
• W2000832493 hasConceptScore W2000832493C2777752497 @default.
• W2000832493 hasConceptScore W2000832493C2778108375 @default.
• W2000832493 hasConceptScore W2000832493C2778563252 @default.
• W2000832493 hasConceptScore W2000832493C2779281246 @default.
• W2000832493 hasConceptScore W2000832493C2780796126 @default.
• W2000832493 hasConceptScore W2000832493C542903549 @default.
• W2000832493 hasConceptScore W2000832493C55493867 @default.
• W2000832493 hasConceptScore W2000832493C71315377 @default.
• W2000832493 hasConceptScore W2000832493C86803240 @default.
• W2000832493 hasConceptScore W2000832493C93984277 @default.
• W2000832493 hasIssue "4" @default.
• W2000832493 hasLocation W20008324931 @default.
• W2000832493 hasLocation W20008324932 @default.
• W2000832493 hasOpenAccess W2000832493 @default.
• W2000832493 hasPrimaryLocation W20008324931 @default.
• W2000832493 hasRelatedWork W1937309249 @default.
• W2000832493 hasRelatedWork W1986631548 @default.
• W2000832493 hasRelatedWork W1988054489 @default.
• W2000832493 hasRelatedWork W1991216500 @default.
• W2000832493 hasRelatedWork W1992084581 @default.
• W2000832493 hasRelatedWork W2000832493 @default.
• W2000832493 hasRelatedWork W2062636887 @default.
• W2000832493 hasRelatedWork W2065569912 @default.
• W2000832493 hasRelatedWork W4379536951 @default.
• W2000832493 hasRelatedWork W166188208 @default.
• W2000832493 hasVolume "11" @default.
• W2000832493 isParatext "false" @default.
• W2000832493 isRetracted "false" @default.
• W2000832493 magId "2000832493" @default.

• next