Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000834887> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2000834887 endingPage "206" @default.
- W2000834887 startingPage "199" @default.
- W2000834887 abstract "Isolated rat hepatocytes attach to, and spread on, the surface of polystyrene tissue culture dishes in the presence of serum. The attachment is essentially complete in 30 min at 37 °C, whereas no attachment occurs at 0 °C. Dead (trypan blue-stainable) cells do not attach; hence the plating efficiency (percentage of cells attaching) is close to the percentage of intact cells in the hepatocyte suspension. Attachment in the presence of serum is relatively independent of pH, but requires divalent cations. Mg2+ stimulates attachment more effectively than Ca2+, and a combination of both cations gives maximal attachment. Cells do not attach readily to untreated dishes in the absence of serum, but attach to and spread on dishes precoated with adsorbed serum protein, concanavalin A (ConA), or a film of collagen. The attachment-promoting activity in serum is destroyed by acid treatment, by heating to 70 °C, and by protease treatment. It is therefore most probably a protein, which, like collagen and ConA, can bind to receptors on the hepatocyte surface." @default.
- W2000834887 created "2016-06-24" @default.
- W2000834887 creator A5029334916 @default.
- W2000834887 creator A5062383478 @default.
- W2000834887 date "1978-10-01" @default.
- W2000834887 modified "2023-10-16" @default.
- W2000834887 title "Attachment of rat hepatocytes in vitro to substrata of serum protein, collagen, or concanavalin A" @default.
- W2000834887 cites W1505071206 @default.
- W2000834887 cites W1510525408 @default.
- W2000834887 cites W1540720368 @default.
- W2000834887 cites W1557370431 @default.
- W2000834887 cites W1565526431 @default.
- W2000834887 cites W1566157726 @default.
- W2000834887 cites W1590377674 @default.
- W2000834887 cites W1605194916 @default.
- W2000834887 cites W1915537365 @default.
- W2000834887 cites W1971548997 @default.
- W2000834887 cites W1985073282 @default.
- W2000834887 cites W1987375895 @default.
- W2000834887 cites W2006126999 @default.
- W2000834887 cites W2007241567 @default.
- W2000834887 cites W2007703421 @default.
- W2000834887 cites W2009241314 @default.
- W2000834887 cites W2011915218 @default.
- W2000834887 cites W2023229316 @default.
- W2000834887 cites W2029934108 @default.
- W2000834887 cites W2046682016 @default.
- W2000834887 cites W2065507421 @default.
- W2000834887 cites W2074877984 @default.
- W2000834887 cites W2076204859 @default.
- W2000834887 cites W2078614733 @default.
- W2000834887 cites W2088468259 @default.
- W2000834887 cites W2093366573 @default.
- W2000834887 cites W2100702098 @default.
- W2000834887 cites W2108358343 @default.
- W2000834887 cites W2108970999 @default.
- W2000834887 cites W2120225939 @default.
- W2000834887 cites W2125146200 @default.
- W2000834887 cites W2141732883 @default.
- W2000834887 cites W2142133226 @default.
- W2000834887 cites W2169275874 @default.
- W2000834887 cites W2182142824 @default.
- W2000834887 cites W4385974830 @default.
- W2000834887 doi "https://doi.org/10.1016/0014-4827(78)90076-9" @default.
- W2000834887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29767" @default.
- W2000834887 hasPublicationYear "1978" @default.
- W2000834887 type Work @default.
- W2000834887 sameAs 2000834887 @default.
- W2000834887 citedByCount "59" @default.
- W2000834887 countsByYear W20008348872013 @default.
- W2000834887 countsByYear W20008348872023 @default.
- W2000834887 crossrefType "journal-article" @default.
- W2000834887 hasAuthorship W2000834887A5029334916 @default.
- W2000834887 hasAuthorship W2000834887A5062383478 @default.
- W2000834887 hasConcept C153911025 @default.
- W2000834887 hasConcept C178790620 @default.
- W2000834887 hasConcept C185592680 @default.
- W2000834887 hasConcept C19869320 @default.
- W2000834887 hasConcept C202751555 @default.
- W2000834887 hasConcept C2776200302 @default.
- W2000834887 hasConcept C2780070996 @default.
- W2000834887 hasConcept C55493867 @default.
- W2000834887 hasConcept C74884574 @default.
- W2000834887 hasConcept C86803240 @default.
- W2000834887 hasConceptScore W2000834887C153911025 @default.
- W2000834887 hasConceptScore W2000834887C178790620 @default.
- W2000834887 hasConceptScore W2000834887C185592680 @default.
- W2000834887 hasConceptScore W2000834887C19869320 @default.
- W2000834887 hasConceptScore W2000834887C202751555 @default.
- W2000834887 hasConceptScore W2000834887C2776200302 @default.
- W2000834887 hasConceptScore W2000834887C2780070996 @default.
- W2000834887 hasConceptScore W2000834887C55493867 @default.
- W2000834887 hasConceptScore W2000834887C74884574 @default.
- W2000834887 hasConceptScore W2000834887C86803240 @default.
- W2000834887 hasIssue "1" @default.
- W2000834887 hasLocation W20008348871 @default.
- W2000834887 hasLocation W20008348872 @default.
- W2000834887 hasOpenAccess W2000834887 @default.
- W2000834887 hasPrimaryLocation W20008348871 @default.
- W2000834887 hasRelatedWork W2000834887 @default.
- W2000834887 hasRelatedWork W2025357993 @default.
- W2000834887 hasRelatedWork W2049449696 @default.
- W2000834887 hasRelatedWork W2074560112 @default.
- W2000834887 hasRelatedWork W2084878274 @default.
- W2000834887 hasRelatedWork W2102735981 @default.
- W2000834887 hasRelatedWork W2424901445 @default.
- W2000834887 hasRelatedWork W2470901852 @default.
- W2000834887 hasRelatedWork W316174557 @default.
- W2000834887 hasRelatedWork W4236389601 @default.
- W2000834887 hasVolume "116" @default.
- W2000834887 isParatext "false" @default.
- W2000834887 isRetracted "false" @default.
- W2000834887 magId "2000834887" @default.
- W2000834887 workType "article" @default.