Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000842995> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2000842995 endingPage "363" @default.
- W2000842995 startingPage "359" @default.
- W2000842995 abstract "As the number of new enteroviruses and human parechoviruses seems ever growing, the necessity for updated diagnostics is relevant. We have updated an enterovirus assay and combined it with a previously published assay for human parechovirus resulting in a multiplex one-step RT-PCR assay. The multiplex assay was validated by analysing the sensitivity and specificity of the assay compared to the respective monoplex assays, and a good concordance was found. Furthermore, the enterovirus assay was able to detect 42 reference strains from all 4 species, and an additional 9 genotypes during panel testing and routine usage. During 15 months of routine use, from October 2008 to December 2009, we received and analysed 2187 samples (stool samples, cerebrospinal fluids, blood samples, respiratory samples and autopsy samples) were tested, from 1546 patients and detected enteroviruses and parechoviruses in 171 (8%) and 66 (3%) of the samples, respectively. 180 of the positive samples could be genotyped by PCR and sequencing and the most common genotypes found were human parechovirus type 3, echovirus 9, enterovirus 71, Coxsackievirus A16, and echovirus 25. During 2009 in Denmark, both enterovirus and human parechovirus type 3 had a similar seasonal pattern with a peak during the summer and autumn. Human parechovirus type 3 was almost invariably found in children less than 4 months of age. In conclusion, a multiplex assay was developed allowing simultaneous detection of 2 viruses, which can cause similar clinical symptoms." @default.
- W2000842995 created "2016-06-24" @default.
- W2000842995 creator A5019275083 @default.
- W2000842995 creator A5020859290 @default.
- W2000842995 creator A5038190558 @default.
- W2000842995 creator A5067260260 @default.
- W2000842995 date "2013-11-01" @default.
- W2000842995 modified "2023-09-26" @default.
- W2000842995 title "A novel enterovirus and parechovirus multiplex one-step real-time PCR-validation and clinical experience" @default.
- W2000842995 cites W2026498900 @default.
- W2000842995 cites W2034662321 @default.
- W2000842995 cites W2034983223 @default.
- W2000842995 cites W2047517181 @default.
- W2000842995 cites W2114799364 @default.
- W2000842995 cites W2132760679 @default.
- W2000842995 cites W2141494827 @default.
- W2000842995 cites W2143101725 @default.
- W2000842995 cites W2146386793 @default.
- W2000842995 cites W2170287831 @default.
- W2000842995 doi "https://doi.org/10.1016/j.jviromet.2013.06.038" @default.
- W2000842995 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7119552" @default.
- W2000842995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23845901" @default.
- W2000842995 hasPublicationYear "2013" @default.
- W2000842995 type Work @default.
- W2000842995 sameAs 2000842995 @default.
- W2000842995 citedByCount "23" @default.
- W2000842995 countsByYear W20008429952014 @default.
- W2000842995 countsByYear W20008429952015 @default.
- W2000842995 countsByYear W20008429952016 @default.
- W2000842995 countsByYear W20008429952017 @default.
- W2000842995 countsByYear W20008429952018 @default.
- W2000842995 countsByYear W20008429952019 @default.
- W2000842995 countsByYear W20008429952021 @default.
- W2000842995 countsByYear W20008429952022 @default.
- W2000842995 countsByYear W20008429952023 @default.
- W2000842995 crossrefType "journal-article" @default.
- W2000842995 hasAuthorship W2000842995A5019275083 @default.
- W2000842995 hasAuthorship W2000842995A5020859290 @default.
- W2000842995 hasAuthorship W2000842995A5038190558 @default.
- W2000842995 hasAuthorship W2000842995A5067260260 @default.
- W2000842995 hasBestOaLocation W20008429951 @default.
- W2000842995 hasConcept C104317684 @default.
- W2000842995 hasConcept C135763542 @default.
- W2000842995 hasConcept C159047783 @default.
- W2000842995 hasConcept C2522874641 @default.
- W2000842995 hasConcept C2777888124 @default.
- W2000842995 hasConcept C2778819740 @default.
- W2000842995 hasConcept C2779120600 @default.
- W2000842995 hasConcept C2781188995 @default.
- W2000842995 hasConcept C49105822 @default.
- W2000842995 hasConcept C54355233 @default.
- W2000842995 hasConcept C86803240 @default.
- W2000842995 hasConcept C89423630 @default.
- W2000842995 hasConcept C90583042 @default.
- W2000842995 hasConceptScore W2000842995C104317684 @default.
- W2000842995 hasConceptScore W2000842995C135763542 @default.
- W2000842995 hasConceptScore W2000842995C159047783 @default.
- W2000842995 hasConceptScore W2000842995C2522874641 @default.
- W2000842995 hasConceptScore W2000842995C2777888124 @default.
- W2000842995 hasConceptScore W2000842995C2778819740 @default.
- W2000842995 hasConceptScore W2000842995C2779120600 @default.
- W2000842995 hasConceptScore W2000842995C2781188995 @default.
- W2000842995 hasConceptScore W2000842995C49105822 @default.
- W2000842995 hasConceptScore W2000842995C54355233 @default.
- W2000842995 hasConceptScore W2000842995C86803240 @default.
- W2000842995 hasConceptScore W2000842995C89423630 @default.
- W2000842995 hasConceptScore W2000842995C90583042 @default.
- W2000842995 hasIssue "2" @default.
- W2000842995 hasLocation W20008429951 @default.
- W2000842995 hasLocation W20008429952 @default.
- W2000842995 hasLocation W20008429953 @default.
- W2000842995 hasLocation W20008429954 @default.
- W2000842995 hasOpenAccess W2000842995 @default.
- W2000842995 hasPrimaryLocation W20008429951 @default.
- W2000842995 hasRelatedWork W1514431218 @default.
- W2000842995 hasRelatedWork W1599532661 @default.
- W2000842995 hasRelatedWork W1987410669 @default.
- W2000842995 hasRelatedWork W2000842995 @default.
- W2000842995 hasRelatedWork W2001317398 @default.
- W2000842995 hasRelatedWork W2003887036 @default.
- W2000842995 hasRelatedWork W2054550444 @default.
- W2000842995 hasRelatedWork W2111259053 @default.
- W2000842995 hasRelatedWork W2123645525 @default.
- W2000842995 hasRelatedWork W4230394199 @default.
- W2000842995 hasVolume "193" @default.
- W2000842995 isParatext "false" @default.
- W2000842995 isRetracted "false" @default.
- W2000842995 magId "2000842995" @default.
- W2000842995 workType "article" @default.