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• W2000847476 abstract "A rapid and highly specific real-time quantitative PCR, based on the amplification of a fragment of the mitochondrial 12S ribosomal RNA gene (rRNA), has been developed for the quantitation of pork (Sus scrofa) in binary pork/beef muscle mixtures. The method combines the use of pork-specific primers, that amplify a 411bp fragment from pork DNA, and mammalian-specific primers amplifying a 425-428bp fragment from mammalian species DNA, which are used as endogenous control. An internal fluorogenic probe (TaqMan), that hybridizes in the pork-specific and also in the mammalian DNA fragments is used to monitor the amplification of the target gene. A comparison of the cycle number (C(t)) at which mammalian and pork-specific PCR products are first detected, in combination with the use of reference standards of known pork content, allows the determination of the percentage of pork in a mixed sample. Analysis of experimental pork/beef muscle binary mixtures demonstrated the specificity and sensitivity of the assay for detection and quantitation of pork in the range 0.5-5%." @default.
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• W2000847476 date "2005-05-01" @default.
• W2000847476 modified "2023-10-03" @default.
• W2000847476 title "TaqMan real-time PCR for the detection and quantitation of pork in meat mixtures" @default.
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• W2000847476 doi "https://doi.org/10.1016/j.meatsci.2004.12.005" @default.
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