Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000848464> ?p ?o ?g. }
- W2000848464 endingPage "3108" @default.
- W2000848464 startingPage "3096" @default.
- W2000848464 abstract "A [2Fe-2S] ferredoxin (Fd1) from the hyperthermophilic bacterium Aquifex aeolicus has been obtained by heterologous expression of the encoding gene in Escherichia coli. Sequence comparisons show that this protein belongs to the extended family of plant- and mammalian-type [2Fe-2S] ferredoxins but also indicate that it is not closely similar to either the plant-type or mammalian-type subfamilies. Instead, it appears to bear some similarity to novel members of this family, in particular the Isc-type ferredoxins involved in the assembly of iron-sulfur clusters in vivo. The two redox levels of the [2Fe-2S](2+/+) metal site of A. aeolicus ferredoxin have been studied by UV-visible, resonance Raman, EPR, variable temperature magnetic circular dichroism, and Mössbauer spectroscopies. A full-spin Hamiltonian analysis is given for the Mössbauer spectra. In aggregate, the spectroscopic data reveal differences with both the plant-type and mammalian-type ferredoxins, in keeping with the sequence comparisons. The midpoint potential of the [2Fe-2S](2+/+) couple, at -375 mV versus the normal hydrogen electrode, is more negative than those of mammalian-type ferredoxins and at the upper end of the range covered by plant-type ferredoxins. A. aeolicus ferredoxin contains two cysteines in addition to the four that are committed as ligands of the [2Fe-2S] cluster. These two residues have been shown by chemical modification and site-directed mutagenesis to form a disulfide bridge in the native protein. While that cystine unit plays a significant role in the exceptional thermostability of A. aeolicus ferredoxin (T(m) = 121 degrees C at pH 7 versus T(m) = 113 degrees C in a molecular variant where the disulfide bridge has been removed), it does not bear on the properties of the [2Fe-2S](2+/+) chromophore. This observation is consistent with the large distance (ca. 20 A) that is predicted to separate the iron-sulfur chromophore from the disulfide bridge." @default.
- W2000848464 created "2016-06-24" @default.
- W2000848464 creator A5009654805 @default.
- W2000848464 creator A5021602538 @default.
- W2000848464 creator A5046997176 @default.
- W2000848464 creator A5058130714 @default.
- W2000848464 creator A5063954863 @default.
- W2000848464 creator A5075937235 @default.
- W2000848464 creator A5086083106 @default.
- W2000848464 date "2002-02-06" @default.
- W2000848464 modified "2023-09-26" @default.
- W2000848464 title "A Hyperthermophilic Plant-Type [2Fe-2S] Ferredoxin from <i>Aquifex aeolicus</i> Is Stabilized by a Disulfide Bond" @default.
- W2000848464 cites W1520439223 @default.
- W2000848464 cites W1544513344 @default.
- W2000848464 cites W1544879095 @default.
- W2000848464 cites W1608218663 @default.
- W2000848464 cites W1788077828 @default.
- W2000848464 cites W1932705370 @default.
- W2000848464 cites W1967503627 @default.
- W2000848464 cites W1976719411 @default.
- W2000848464 cites W1994264299 @default.
- W2000848464 cites W1998610986 @default.
- W2000848464 cites W2010056390 @default.
- W2000848464 cites W2013835300 @default.
- W2000848464 cites W2029772331 @default.
- W2000848464 cites W2034394080 @default.
- W2000848464 cites W2034402926 @default.
- W2000848464 cites W2039383448 @default.
- W2000848464 cites W2042452521 @default.
- W2000848464 cites W2042929656 @default.
- W2000848464 cites W2044852484 @default.
- W2000848464 cites W2062232288 @default.
- W2000848464 cites W2082920672 @default.
- W2000848464 cites W2094593177 @default.
- W2000848464 cites W2137993665 @default.
- W2000848464 cites W2166964423 @default.
- W2000848464 cites W2202902095 @default.
- W2000848464 cites W4239629847 @default.
- W2000848464 doi "https://doi.org/10.1021/bi015981m" @default.
- W2000848464 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11863449" @default.
- W2000848464 hasPublicationYear "2002" @default.
- W2000848464 type Work @default.
- W2000848464 sameAs 2000848464 @default.
- W2000848464 citedByCount "56" @default.
- W2000848464 countsByYear W20008484642012 @default.
- W2000848464 countsByYear W20008484642013 @default.
- W2000848464 countsByYear W20008484642014 @default.
- W2000848464 countsByYear W20008484642015 @default.
- W2000848464 countsByYear W20008484642016 @default.
- W2000848464 countsByYear W20008484642017 @default.
- W2000848464 countsByYear W20008484642019 @default.
- W2000848464 countsByYear W20008484642020 @default.
- W2000848464 countsByYear W20008484642022 @default.
- W2000848464 countsByYear W20008484642023 @default.
- W2000848464 crossrefType "journal-article" @default.
- W2000848464 hasAuthorship W2000848464A5009654805 @default.
- W2000848464 hasAuthorship W2000848464A5021602538 @default.
- W2000848464 hasAuthorship W2000848464A5046997176 @default.
- W2000848464 hasAuthorship W2000848464A5058130714 @default.
- W2000848464 hasAuthorship W2000848464A5063954863 @default.
- W2000848464 hasAuthorship W2000848464A5075937235 @default.
- W2000848464 hasAuthorship W2000848464A5086083106 @default.
- W2000848464 hasConcept C104317684 @default.
- W2000848464 hasConcept C133571119 @default.
- W2000848464 hasConcept C134621786 @default.
- W2000848464 hasConcept C181199279 @default.
- W2000848464 hasConcept C185592680 @default.
- W2000848464 hasConcept C2777400929 @default.
- W2000848464 hasConcept C2777737464 @default.
- W2000848464 hasConcept C3623737 @default.
- W2000848464 hasConcept C547475151 @default.
- W2000848464 hasConcept C55493867 @default.
- W2000848464 hasConcept C70412867 @default.
- W2000848464 hasConcept C71240020 @default.
- W2000848464 hasConcept C8010536 @default.
- W2000848464 hasConceptScore W2000848464C104317684 @default.
- W2000848464 hasConceptScore W2000848464C133571119 @default.
- W2000848464 hasConceptScore W2000848464C134621786 @default.
- W2000848464 hasConceptScore W2000848464C181199279 @default.
- W2000848464 hasConceptScore W2000848464C185592680 @default.
- W2000848464 hasConceptScore W2000848464C2777400929 @default.
- W2000848464 hasConceptScore W2000848464C2777737464 @default.
- W2000848464 hasConceptScore W2000848464C3623737 @default.
- W2000848464 hasConceptScore W2000848464C547475151 @default.
- W2000848464 hasConceptScore W2000848464C55493867 @default.
- W2000848464 hasConceptScore W2000848464C70412867 @default.
- W2000848464 hasConceptScore W2000848464C71240020 @default.
- W2000848464 hasConceptScore W2000848464C8010536 @default.
- W2000848464 hasIssue "9" @default.
- W2000848464 hasLocation W20008484641 @default.
- W2000848464 hasLocation W20008484642 @default.
- W2000848464 hasOpenAccess W2000848464 @default.
- W2000848464 hasPrimaryLocation W20008484641 @default.
- W2000848464 hasRelatedWork W2000848464 @default.
- W2000848464 hasRelatedWork W2003011549 @default.
- W2000848464 hasRelatedWork W2013594387 @default.
- W2000848464 hasRelatedWork W2044874189 @default.
- W2000848464 hasRelatedWork W2058038709 @default.