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- W2000850827 abstract "Abstract The messenger RNA species specified by Sindbis virus in infected cells, 26 and 42 S RNA, have been characterized with regard to their extreme 5′-terminal sequences. ( Methyl - 3 H)-Labeled m 7 G in “caps” was used to detect cap-containing, and hence 5′-terminal, oligonucleotides after digestion with ribonuclease A, or ribonuclease T 1 , plus phosphatase. Digests were fractionated by DEAE column chromatography, by cellulose acetate electrophoresis, and by DEAE paper electrophoresis. The latter two systems were also applied sequentially to 32 P-labeled samples to generate oligonucleotide fingerprints. The cap-containing oligonucleotide from T 1 -plus-phosphatase digests of 26 S RNA was isolated and shown to be m 7 GpppApUpG. The homologous oligonucleotide from such a digest of 42 S RNA behaved as if it were one pyrimidine residue larger, whereas cap-containing oligonucleotides from ribonuclease A-plus-phosphatase digests of the two RNA species had identical chromatographic and electrophoretic properties. We infer from these and earlier findings that Sindbis virus-specified 42 S RNA terminates in m 7 GpppApUpYpG. These results support the idea that 26 S RNA and 42 S RNA arise from two distinct and different initiation sites." @default.
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- W2000850827 date "1979-10-01" @default.
- W2000850827 modified "2023-10-17" @default.
- W2000850827 title "The extreme 5′-terminal sequences of sindbis virus 26 and 42 S RNA" @default.
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- W2000850827 doi "https://doi.org/10.1016/0042-6822(79)90532-4" @default.
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