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- W2000852925 abstract "Background: We previously reported that an attenuated adenovirus (OBP-301, ‘Telomelysin’), in which the hTERT promoter element drives expression of E1A and E1B genes linked with an IRES, could replicate in cancer cells, and causes selective lysis of cancer cells. In this study, we further investigated the virus replication and antitumor activity of Telomelysin in various human cancer cell lines, and also established the assay to determine the concentration of Telomelysin in circulating blood. Methods: We designed the specific primer targeted for E1 gene or IRES sequence for real-time quantitative PCR (Q-PCR), and then analyzed its potent of quantity and sensitivity. We also analyzed the correlation between hTERT mRNA expression and viral replication of Telomelysin in human cancer cells. CAR expression was determined by flow cytometry, and cell killing effect of Telomelysin in 13 human cancer cell lines and normal human fibroblasts was examined by XTT assay. Results: By Q-PCR, the minimum detection limit was 100 plaque- forming units (PFU)/ml of Telomelysin in the plasma, using the primer for IRES. A positive correlation tendency was observed between hTERT mRNA expression and viral replication of Telomelysin in human cancer cells. Telomelysin killed all human cancer cell lines dose-dependently, but not normal human fibroblasts. Average of ID50 after three days of infection was 12.1 multiplicity of infection (MOI). Conclusion: Our data suggested that Telomelysin may be useful for treatment of various human cancer cells, and telomerase activity is a marker for Telomelysin replication in cancer cells." @default.
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- W2000852925 date "2006-01-01" @default.
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- W2000852925 title "143. Development of Telomerase Specific Antineoplastic Adenovirus Telomelysin (OBP-301): Establishment of Useful Assay and Assessment for Antitumor Activity" @default.
- W2000852925 doi "https://doi.org/10.1016/j.ymthe.2006.08.164" @default.
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