Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000855407> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2000855407 endingPage "1330" @default.
- W2000855407 startingPage "1326" @default.
- W2000855407 abstract "Streptococcus pneumoniae is a major cause of bacteremia, meningitis, pneumonia, sinusitis, and acute otitis media in children. Although optochin susceptibility, bile solubility, and Quellung testing are the standards for identifying and differentiating pneumococci, there are several reports of nontypeable pneumococci that give inconsistent results with one or more of these tests. We characterized 52 isolates previously labeled as nontypeable pneumococci. Microbiological methods included repeating the Quellung reaction using a new and expanded group of antisera, optochin susceptibility and bile solubility tests, and automated Vitek 2 identification. Molecular methods included PCR detection of ply and psaA genes, multilocus sequence typing (MLST), 16S rRNA gene sequencing, and pyrosequencing. Of the 52 isolates, 38 (73%) were optochin susceptible, were psaA and ply positive, and could be serotyped by the Quellung reaction. The remaining 14 isolates, isolated from patients with otitis media (n = 6), bacteremia (n = 6), meningitis (n = 1), and pneumonia (n = 1), underwent further analysis. Three of these 14 isolates were nontypeable due to autoagglutination but were pneumococci by all tests and represented pneumococcal sequence types previously recognized by MLST. The 11 remaining isolates were optochin resistant, and 6 of these were bile soluble. Three of 11 were both psaA and ply positive and clustered with pneumococci by MLST (2 were bile soluble); 8 lacked psaA (5 ply positive, 4 bile soluble) and likely belonged to other Streptococcus species. In conclusion, few isolates were truly nontypeable by Quellung reaction, and MLST and the presence of psaA proved useful in distinguishing between atypical pneumococci and other streptococcal species." @default.
- W2000855407 created "2016-06-24" @default.
- W2000855407 creator A5002448964 @default.
- W2000855407 creator A5018304808 @default.
- W2000855407 creator A5027953175 @default.
- W2000855407 creator A5039303050 @default.
- W2000855407 creator A5054604363 @default.
- W2000855407 creator A5061273099 @default.
- W2000855407 creator A5084936395 @default.
- W2000855407 date "2012-01-11" @default.
- W2000855407 modified "2023-09-24" @default.
- W2000855407 title "Characterization of Nontypeable and Atypical Streptococcus pneumoniae Pediatric Isolates from 1994 to 2010" @default.
- W2000855407 cites W2000499723 @default.
- W2000855407 cites W2010452458 @default.
- W2000855407 cites W2012118784 @default.
- W2000855407 cites W2013572910 @default.
- W2000855407 cites W2023964275 @default.
- W2000855407 cites W2048721933 @default.
- W2000855407 cites W2064505667 @default.
- W2000855407 cites W2079603390 @default.
- W2000855407 cites W2094444073 @default.
- W2000855407 cites W2107096230 @default.
- W2000855407 cites W2108130630 @default.
- W2000855407 cites W2109882926 @default.
- W2000855407 cites W2110713840 @default.
- W2000855407 cites W2111775329 @default.
- W2000855407 cites W2132632499 @default.
- W2000855407 cites W2136238217 @default.
- W2000855407 cites W2141992911 @default.
- W2000855407 cites W2159427865 @default.
- W2000855407 doi "https://doi.org/10.1128/jcm.05182-11" @default.
- W2000855407 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3318545" @default.
- W2000855407 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22238440" @default.
- W2000855407 hasPublicationYear "2012" @default.
- W2000855407 type Work @default.
- W2000855407 sameAs 2000855407 @default.
- W2000855407 citedByCount "15" @default.
- W2000855407 countsByYear W20008554072012 @default.
- W2000855407 countsByYear W20008554072013 @default.
- W2000855407 countsByYear W20008554072015 @default.
- W2000855407 countsByYear W20008554072016 @default.
- W2000855407 countsByYear W20008554072017 @default.
- W2000855407 countsByYear W20008554072019 @default.
- W2000855407 countsByYear W20008554072020 @default.
- W2000855407 crossrefType "journal-article" @default.
- W2000855407 hasAuthorship W2000855407A5002448964 @default.
- W2000855407 hasAuthorship W2000855407A5018304808 @default.
- W2000855407 hasAuthorship W2000855407A5027953175 @default.
- W2000855407 hasAuthorship W2000855407A5039303050 @default.
- W2000855407 hasAuthorship W2000855407A5054604363 @default.
- W2000855407 hasAuthorship W2000855407A5061273099 @default.
- W2000855407 hasAuthorship W2000855407A5084936395 @default.
- W2000855407 hasBestOaLocation W20008554071 @default.
- W2000855407 hasConcept C104317684 @default.
- W2000855407 hasConcept C135763542 @default.
- W2000855407 hasConcept C2778276568 @default.
- W2000855407 hasConcept C2779443120 @default.
- W2000855407 hasConcept C2781253189 @default.
- W2000855407 hasConcept C501593827 @default.
- W2000855407 hasConcept C523546767 @default.
- W2000855407 hasConcept C54355233 @default.
- W2000855407 hasConcept C77434933 @default.
- W2000855407 hasConcept C86803240 @default.
- W2000855407 hasConcept C89423630 @default.
- W2000855407 hasConceptScore W2000855407C104317684 @default.
- W2000855407 hasConceptScore W2000855407C135763542 @default.
- W2000855407 hasConceptScore W2000855407C2778276568 @default.
- W2000855407 hasConceptScore W2000855407C2779443120 @default.
- W2000855407 hasConceptScore W2000855407C2781253189 @default.
- W2000855407 hasConceptScore W2000855407C501593827 @default.
- W2000855407 hasConceptScore W2000855407C523546767 @default.
- W2000855407 hasConceptScore W2000855407C54355233 @default.
- W2000855407 hasConceptScore W2000855407C77434933 @default.
- W2000855407 hasConceptScore W2000855407C86803240 @default.
- W2000855407 hasConceptScore W2000855407C89423630 @default.
- W2000855407 hasIssue "4" @default.
- W2000855407 hasLocation W20008554071 @default.
- W2000855407 hasLocation W20008554072 @default.
- W2000855407 hasLocation W20008554073 @default.
- W2000855407 hasLocation W20008554074 @default.
- W2000855407 hasOpenAccess W2000855407 @default.
- W2000855407 hasPrimaryLocation W20008554071 @default.
- W2000855407 hasRelatedWork W2000855407 @default.
- W2000855407 hasRelatedWork W2010246334 @default.
- W2000855407 hasRelatedWork W2030804603 @default.
- W2000855407 hasRelatedWork W2056442393 @default.
- W2000855407 hasRelatedWork W2084807013 @default.
- W2000855407 hasRelatedWork W2141992911 @default.
- W2000855407 hasRelatedWork W2381100072 @default.
- W2000855407 hasRelatedWork W2460529062 @default.
- W2000855407 hasRelatedWork W3035289353 @default.
- W2000855407 hasRelatedWork W4206604187 @default.
- W2000855407 hasVolume "50" @default.
- W2000855407 isParatext "false" @default.
- W2000855407 isRetracted "false" @default.
- W2000855407 magId "2000855407" @default.
- W2000855407 workType "article" @default.