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• W2000862652 abstract "Contents Cumulus‐oocyte complexes from hormone‐stimulated 3–4‐week‐old (n = 43) and 6–7‐week‐old (n = 12) prepubertal lambs were matured in vitro and incubated with unsorted, or X‐ or Y‐spermatozoa separated with a high‐speed cell sorter (SX MoFlo ® ) frozen‐thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p < 0.05) with unsorted (515/926, 55.6%) than X‐ or Y‐spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p < 0.05) for X‐ (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y‐spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X‐ and Y‐spermatozoa (p > 0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X‐ and 16.7% (1/6) for Y‐spermatozoa‐derived embryos (p > 0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X‐ and 2.9% (1/34) Y‐spermatozoa‐derived embryos (p > 0.05). Of the two lambs born from X‐spermatozoa‐derived embryos, one was female (50%), and from the two Y‐spermatozoa‐derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen‐thawed sex‐sorted sperm." @default.
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• W2000862652 date "2004-12-01" @default.
• W2000862652 modified "2023-10-15" @default.
• W2000862652 title "Production of Lambs After the Transfer of Fresh and Cryopreserved <i>in vitro</i> Produced Embryos from Prepubertal Lamb Oocytes and Unsorted and Sex‐sorted Frozen‐thawed Spermatozoa" @default.
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• W2000862652 doi "https://doi.org/10.1111/j.1439-0531.2004.00542.x" @default.
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