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- W2000868032 abstract "Metaphase chromosomes were obtained from cell suspension cultures of Haplopappus gracilis (Nutt.) Gray using a mass isolation procedure, and were subsequently sorted by flow cytometry. The integrity of the sorted chromosomes, isolated at neutral pH, was examined by electron microscopy and DNA analysis. Electron microscopy showed no significant damage in the DNA leaked out of the chromosomes at high pH. Sedimentation of chromosomal DNA in alkaline sucrose gradients showed the presence of high molecular weight single stranded DNA fragments (mean MW 4–20 × 108 daltons). These characteristics indicate preservation of DNA integrity and general chromosome structure during the synchronization of the cell cycle, the preparation of protoplasts and the subsequent chromosome isolation procedures. Implications for the use of such chromosomes for genetic manipulation are discussed." @default.
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- W2000868032 date "1986-01-01" @default.
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- W2000868032 title "The integrity of metaphase chromosomes of Haplopappus gracilis (Nutt.) gray isolated by flow cytometry" @default.
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- W2000868032 doi "https://doi.org/10.1016/0168-9452(86)90061-0" @default.
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