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- W2000872074 abstract "In view of the pivotal role played by the diversity of fatty acid-derived oxy-products in a vast array of physiological processes, precise knowledge about the molecular principles dictating substrate specificity and regioselectivity in P450-catalyzed oxidative attack on the distinctly structured carbon chains of the monocarboxylic acids is of paramount importance. Based on a general, CYP102A1- related construct, the majority of prospective key determinants participating in fatty acid recognition/binding were found to cluster near the distal heme face made up by the helical B, F, G and I tetrad as well as the B-C interhelical loop and certain β-sheet segments. Most of the contact sites examined show a frequency of conservation < 10%, hinting at the requirement of some degree of conformational flexibility. Some decisive elements may also have a function in maintaining active-site integrity, governing substrate access to the catalytic centre, and steering the redox machinery to efficiently promote fatty acid oxidations. Physico-chemical factors imposing constraints on orientation of the fatty acid molecules towards the iron-oxene core focus on the variably expressed polarity profile of the diverse docking regions and bulkiness of critical amino acid side chains, acting as selectivity filters for the substrate homologues. Also, dynamic fluctuations of certain contact sites located in the distal backbone of P450s may impact fatty acid positioning. Genetic engineering to introduce versatile properties into fatty acid hydroxylases may give an impetus to biotechnological exploitation of the tailored enzymes in the production of fine chemicals and therapeutic agents. Keywords: P450, Fatty acids, Substrate docking, Catalytic oxidations" @default.
- W2000872074 created "2016-06-24" @default.
- W2000872074 creator A5009261003 @default.
- W2000872074 creator A5040512195 @default.
- W2000872074 date "2010-01-01" @default.
- W2000872074 modified "2023-09-27" @default.
- W2000872074 title "Oxidative Biotransformation of Fatty Acids by Cytochromes P450: Predicted Key Structural Elements Orchestrating Substrate Specificity, Regioselectivity and Catalytic Efficiency" @default.
- W2000872074 cites W113300012 @default.
- W2000872074 cites W1491103665 @default.
- W2000872074 cites W1493462141 @default.
- W2000872074 cites W1495151627 @default.
- W2000872074 cites W1498191276 @default.
- W2000872074 cites W1498652161 @default.
- W2000872074 cites W1503118320 @default.
- W2000872074 cites W1533372435 @default.
- W2000872074 cites W1537833276 @default.
- W2000872074 cites W1542993726 @default.
- W2000872074 cites W1549849157 @default.
- W2000872074 cites W1588262878 @default.
- W2000872074 cites W1595207767 @default.
- W2000872074 cites W1599525955 @default.
- W2000872074 cites W1606963275 @default.
- W2000872074 cites W1607139650 @default.
- W2000872074 cites W1771489119 @default.
- W2000872074 cites W181254387 @default.
- W2000872074 cites W1963820859 @default.
- W2000872074 cites W1964485247 @default.
- W2000872074 cites W1965560431 @default.
- W2000872074 cites W1966066863 @default.
- W2000872074 cites W1970701255 @default.
- W2000872074 cites W1971576421 @default.
- W2000872074 cites W1971866201 @default.
- W2000872074 cites W1972023813 @default.
- W2000872074 cites W1973247586 @default.
- W2000872074 cites W1973549626 @default.
- W2000872074 cites W1974181723 @default.
- W2000872074 cites W1975630478 @default.
- W2000872074 cites W1980170591 @default.
- W2000872074 cites W1980334177 @default.
- W2000872074 cites W1980718944 @default.
- W2000872074 cites W1981410098 @default.
- W2000872074 cites W1982005737 @default.
- W2000872074 cites W1982172626 @default.
- W2000872074 cites W1982426806 @default.
- W2000872074 cites W1987246053 @default.
- W2000872074 cites W1987491348 @default.
- W2000872074 cites W1987902377 @default.
- W2000872074 cites W198886960 @default.
- W2000872074 cites W1989272963 @default.
- W2000872074 cites W1992790976 @default.
- W2000872074 cites W1992879486 @default.
- W2000872074 cites W199420752 @default.
- W2000872074 cites W1996194491 @default.
- W2000872074 cites W1999419099 @default.
- W2000872074 cites W2000051743 @default.
- W2000872074 cites W2000453037 @default.
- W2000872074 cites W2001997090 @default.
- W2000872074 cites W2002194538 @default.
- W2000872074 cites W2002804207 @default.
- W2000872074 cites W2003101947 @default.
- W2000872074 cites W2003105919 @default.
- W2000872074 cites W2004718408 @default.
- W2000872074 cites W2004972021 @default.
- W2000872074 cites W2005742461 @default.
- W2000872074 cites W2006998778 @default.
- W2000872074 cites W2007230769 @default.
- W2000872074 cites W2007321993 @default.
- W2000872074 cites W2010164223 @default.
- W2000872074 cites W2011249023 @default.
- W2000872074 cites W2013999949 @default.
- W2000872074 cites W2017474351 @default.
- W2000872074 cites W2018561455 @default.
- W2000872074 cites W2018961522 @default.
- W2000872074 cites W2019308980 @default.
- W2000872074 cites W2021321407 @default.
- W2000872074 cites W2021853164 @default.
- W2000872074 cites W2021953523 @default.
- W2000872074 cites W2022471249 @default.
- W2000872074 cites W2029758351 @default.
- W2000872074 cites W2030539641 @default.
- W2000872074 cites W2032890964 @default.
- W2000872074 cites W2032952862 @default.
- W2000872074 cites W2034632234 @default.
- W2000872074 cites W2036571037 @default.
- W2000872074 cites W2036732403 @default.
- W2000872074 cites W2046017178 @default.
- W2000872074 cites W2047708413 @default.
- W2000872074 cites W2048498604 @default.
- W2000872074 cites W2050079216 @default.
- W2000872074 cites W2052607746 @default.
- W2000872074 cites W2053554181 @default.
- W2000872074 cites W2054538965 @default.
- W2000872074 cites W2054783858 @default.
- W2000872074 cites W2056119097 @default.
- W2000872074 cites W2058252718 @default.
- W2000872074 cites W2061386796 @default.
- W2000872074 cites W2061850722 @default.
- W2000872074 cites W2062208322 @default.