FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000873083> ?p ?o ?g. }

• W2000873083 endingPage "47" @default.
• W2000873083 startingPage "42" @default.
• W2000873083 abstract "The turnover numbers and other kinetic constants for human alcohol dehydrogenase (ADH) 4 (“stomach” isoenzyme) are substantially larger (10–100-fold) than those for human class I and horse liver alcohol dehydrogenases. Comparison of the primary amino acid sequences (69% identity) and tertiary structures of these enzymes led to the suggestion that residue 317, which makes a hydrogen bond with the nicotinamide amide nitrogen of the coenzyme, may account for these differences. Ala-317 in the class I enzymes is substituted with Cys in human ADH4, and locally different conformations of the peptide backbones could affect coenzyme binding. This hypothesis was tested by making the A317C substitution in horse liver ADH1E and comparisons to the wild-type ADH1E. The steady-state kinetic constants for the oxidation of benzyl alcohol and the reduction of benzaldehyde catalyzed by the A317C enzyme were very similar (up to about 2-fold differences) to those for the wild-type enzyme. Transient kinetics showed that the rate constants for binding of NAD+ and NADH were also similar. Transient reaction data were fitted to the full Ordered Bi Bi mechanism and showed that the rate constants for hydride transfer decreased by about 2.8-fold with the A317C substitution. The structure of A317C ADH1E complexed with NAD+ and 2,3,4,5,6-pentafluorobenzyl alcohol at 1.2 Å resolution is essentially identical to the structure of the wild-type enzyme, except near residue 317 where the additional sulfhydryl group displaces a water molecule that is present in the wild-type enzyme. ADH is adaptable and can tolerate internal substitutions, but the protein dynamics apparently are affected, as reflected in rates of hydride transfer. The A317C substitution is not solely responsible for the larger kinetic constants in human ADH4; thus, the differences in catalytic activity must arise from one or more of the other hundred substitutions in the enzyme." @default.
• W2000873083 created "2016-06-24" @default.
• W2000873083 creator A5015206461 @default.
• W2000873083 creator A5048311352 @default.
• W2000873083 date "2011-05-01" @default.
• W2000873083 modified "2023-09-25" @default.
• W2000873083 title "Origins of the high catalytic activity of human alcohol dehydrogenase 4 studied with horse liver A317C alcohol dehydrogenase" @default.
• W2000873083 cites W1189536871 @default.
• W2000873083 cites W1500082482 @default.
• W2000873083 cites W1525724539 @default.
• W2000873083 cites W1544860800 @default.
• W2000873083 cites W1552270283 @default.
• W2000873083 cites W1569170977 @default.
• W2000873083 cites W1584814136 @default.
• W2000873083 cites W1586079162 @default.
• W2000873083 cites W1606021311 @default.
• W2000873083 cites W1966779021 @default.
• W2000873083 cites W1971176983 @default.
• W2000873083 cites W1976289352 @default.
• W2000873083 cites W1976836655 @default.
• W2000873083 cites W1982281493 @default.
• W2000873083 cites W1986191025 @default.
• W2000873083 cites W1987853577 @default.
• W2000873083 cites W1989543688 @default.
• W2000873083 cites W1993702476 @default.
• W2000873083 cites W1998885514 @default.
• W2000873083 cites W2008428704 @default.
• W2000873083 cites W2008603784 @default.
• W2000873083 cites W2010162199 @default.
• W2000873083 cites W2013083986 @default.
• W2000873083 cites W2015171280 @default.
• W2000873083 cites W2015902468 @default.
• W2000873083 cites W2018154356 @default.
• W2000873083 cites W2025704648 @default.
• W2000873083 cites W2026334274 @default.
• W2000873083 cites W2029781817 @default.
• W2000873083 cites W2038840577 @default.
• W2000873083 cites W2041916914 @default.
• W2000873083 cites W2048730019 @default.
• W2000873083 cites W2059741578 @default.
• W2000873083 cites W2065606310 @default.
• W2000873083 cites W2080658778 @default.
• W2000873083 cites W2087534166 @default.
• W2000873083 cites W2096460115 @default.
• W2000873083 cites W2116947993 @default.
• W2000873083 cites W2167081335 @default.
• W2000873083 cites W2969273703 @default.
• W2000873083 doi "https://doi.org/10.1016/j.cbi.2010.12.015" @default.
• W2000873083 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3103598" @default.
• W2000873083 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21184752" @default.
• W2000873083 hasPublicationYear "2011" @default.
• W2000873083 type Work @default.
• W2000873083 sameAs 2000873083 @default.
• W2000873083 citedByCount "13" @default.
• W2000873083 countsByYear W20008730832012 @default.
• W2000873083 countsByYear W20008730832013 @default.
• W2000873083 countsByYear W20008730832014 @default.
• W2000873083 countsByYear W20008730832016 @default.
• W2000873083 countsByYear W20008730832017 @default.
• W2000873083 countsByYear W20008730832018 @default.
• W2000873083 countsByYear W20008730832019 @default.
• W2000873083 countsByYear W20008730832022 @default.
• W2000873083 countsByYear W20008730832023 @default.
• W2000873083 crossrefType "journal-article" @default.
• W2000873083 hasAuthorship W2000873083A5015206461 @default.
• W2000873083 hasAuthorship W2000873083A5048311352 @default.
• W2000873083 hasBestOaLocation W20008730832 @default.
• W2000873083 hasConcept C121332964 @default.
• W2000873083 hasConcept C148898269 @default.
• W2000873083 hasConcept C181199279 @default.
• W2000873083 hasConcept C185592680 @default.
• W2000873083 hasConcept C197957613 @default.
• W2000873083 hasConcept C2777344606 @default.
• W2000873083 hasConcept C2779728014 @default.
• W2000873083 hasConcept C2781066024 @default.
• W2000873083 hasConcept C55493867 @default.
• W2000873083 hasConcept C62520636 @default.
• W2000873083 hasConcept C71240020 @default.
• W2000873083 hasConcept C75520062 @default.
• W2000873083 hasConcept C93391505 @default.
• W2000873083 hasConceptScore W2000873083C121332964 @default.
• W2000873083 hasConceptScore W2000873083C148898269 @default.
• W2000873083 hasConceptScore W2000873083C181199279 @default.
• W2000873083 hasConceptScore W2000873083C185592680 @default.
• W2000873083 hasConceptScore W2000873083C197957613 @default.
• W2000873083 hasConceptScore W2000873083C2777344606 @default.
• W2000873083 hasConceptScore W2000873083C2779728014 @default.
• W2000873083 hasConceptScore W2000873083C2781066024 @default.
• W2000873083 hasConceptScore W2000873083C55493867 @default.
• W2000873083 hasConceptScore W2000873083C62520636 @default.
• W2000873083 hasConceptScore W2000873083C71240020 @default.
• W2000873083 hasConceptScore W2000873083C75520062 @default.
• W2000873083 hasConceptScore W2000873083C93391505 @default.
• W2000873083 hasIssue "1-3" @default.
• W2000873083 hasLocation W20008730831 @default.
• W2000873083 hasLocation W20008730832 @default.
• W2000873083 hasLocation W20008730833 @default.
• W2000873083 hasLocation W20008730834 @default.

• next