Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000873780> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2000873780 abstract "We developed a simple, tiny setup with only a few optical devices for fast and sensitive identification of DNA with a short-pulse semiconductor laser operating at 20 MHz. In combination with newly synthesized fluorescent dyes (rhodamine derivatives) which exhibit high fluorescence quantum efficiencies and distinct fluorescence lifetimes at semiconductor laser excitation wavelength a sensitive detection and time-resolved identification of DNA can be achieved. At an excitation wavelength of 635 nm the fluorescence background is greatly reduced. We demonstrate the DNA identification of A- and G-terminated DNA fragments labeled at the 5'-end with the rhodamine derivatives MR 200- 1 and JA 169 during capillary gel electrophoresis. The characteristic time-resolved data are acquired by the time- correlated single-photon counting technique. Time-resolved identification analysis is realized by the maximum likelihood estimator. For prediction of the error rate (misclassification) Cramers equation in combination with a pattern recognition technique is applied. These methods deliver high reliabilities at low classification error rates for low fluorescence light level applications." @default.
- W2000873780 created "2016-06-24" @default.
- W2000873780 creator A5006887252 @default.
- W2000873780 creator A5016966519 @default.
- W2000873780 creator A5028796159 @default.
- W2000873780 creator A5048070356 @default.
- W2000873780 creator A5048428962 @default.
- W2000873780 creator A5056428016 @default.
- W2000873780 creator A5057281562 @default.
- W2000873780 creator A5057305305 @default.
- W2000873780 creator A5078030919 @default.
- W2000873780 date "1997-05-07" @default.
- W2000873780 modified "2023-09-23" @default.
- W2000873780 title "<title>Time-resolved DNA identification in capillary gel electrophoresis with semiconductor lasers</title>" @default.
- W2000873780 doi "https://doi.org/10.1117/12.273569" @default.
- W2000873780 hasPublicationYear "1997" @default.
- W2000873780 type Work @default.
- W2000873780 sameAs 2000873780 @default.
- W2000873780 citedByCount "5" @default.
- W2000873780 crossrefType "proceedings-article" @default.
- W2000873780 hasAuthorship W2000873780A5006887252 @default.
- W2000873780 hasAuthorship W2000873780A5016966519 @default.
- W2000873780 hasAuthorship W2000873780A5028796159 @default.
- W2000873780 hasAuthorship W2000873780A5048070356 @default.
- W2000873780 hasAuthorship W2000873780A5048428962 @default.
- W2000873780 hasAuthorship W2000873780A5056428016 @default.
- W2000873780 hasAuthorship W2000873780A5057281562 @default.
- W2000873780 hasAuthorship W2000873780A5057305305 @default.
- W2000873780 hasAuthorship W2000873780A5078030919 @default.
- W2000873780 hasConcept C108225325 @default.
- W2000873780 hasConcept C113196181 @default.
- W2000873780 hasConcept C120665830 @default.
- W2000873780 hasConcept C121332964 @default.
- W2000873780 hasConcept C1342733 @default.
- W2000873780 hasConcept C161790260 @default.
- W2000873780 hasConcept C185592680 @default.
- W2000873780 hasConcept C192562407 @default.
- W2000873780 hasConcept C2776002898 @default.
- W2000873780 hasConcept C2779916640 @default.
- W2000873780 hasConcept C2900643 @default.
- W2000873780 hasConcept C43617362 @default.
- W2000873780 hasConcept C49040817 @default.
- W2000873780 hasConcept C520434653 @default.
- W2000873780 hasConcept C55493867 @default.
- W2000873780 hasConcept C65165184 @default.
- W2000873780 hasConcept C91881484 @default.
- W2000873780 hasConceptScore W2000873780C108225325 @default.
- W2000873780 hasConceptScore W2000873780C113196181 @default.
- W2000873780 hasConceptScore W2000873780C120665830 @default.
- W2000873780 hasConceptScore W2000873780C121332964 @default.
- W2000873780 hasConceptScore W2000873780C1342733 @default.
- W2000873780 hasConceptScore W2000873780C161790260 @default.
- W2000873780 hasConceptScore W2000873780C185592680 @default.
- W2000873780 hasConceptScore W2000873780C192562407 @default.
- W2000873780 hasConceptScore W2000873780C2776002898 @default.
- W2000873780 hasConceptScore W2000873780C2779916640 @default.
- W2000873780 hasConceptScore W2000873780C2900643 @default.
- W2000873780 hasConceptScore W2000873780C43617362 @default.
- W2000873780 hasConceptScore W2000873780C49040817 @default.
- W2000873780 hasConceptScore W2000873780C520434653 @default.
- W2000873780 hasConceptScore W2000873780C55493867 @default.
- W2000873780 hasConceptScore W2000873780C65165184 @default.
- W2000873780 hasConceptScore W2000873780C91881484 @default.
- W2000873780 hasLocation W20008737801 @default.
- W2000873780 hasOpenAccess W2000873780 @default.
- W2000873780 hasPrimaryLocation W20008737801 @default.
- W2000873780 hasRelatedWork W1970570574 @default.
- W2000873780 hasRelatedWork W2022778450 @default.
- W2000873780 hasRelatedWork W2041977691 @default.
- W2000873780 hasRelatedWork W2047633423 @default.
- W2000873780 hasRelatedWork W2051581688 @default.
- W2000873780 hasRelatedWork W2062750886 @default.
- W2000873780 hasRelatedWork W2072322544 @default.
- W2000873780 hasRelatedWork W2099217060 @default.
- W2000873780 hasRelatedWork W2384385663 @default.
- W2000873780 hasRelatedWork W4206854575 @default.
- W2000873780 isParatext "false" @default.
- W2000873780 isRetracted "false" @default.
- W2000873780 magId "2000873780" @default.
- W2000873780 workType "article" @default.