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• W2000878181 abstract "Abstract Metalloproteomics is a rapidly developing field of science that involves the comprehensive analysis of all metal‐containing or metal‐binding proteins in a biological sample. The purpose of this review is to offer an overview of the research involving Metal Isotope native RadioAutography in Gel Electrophoresis (MIRAGE), a powerful new method to visualize and study the proteome of a particular metal ion. MIRAGE involves four steps: i ) labelling of target proteins with a radioisotope; ii ) separation of intact holo‐proteins using native isoelectric focusing (1D) combined with Blue Native PAGE (2D); iii ) spot visualization and quantification using autoradiography; and iv ) protein identification by tandem mass spectrometry. MIRAGE Investigations of the soluble Cu, Zn, and Fe metalloproteomes of Escherichia coli , and of the soluble Mo and W proteomes of the hyperthermophilic archaeon Pyrococcus furiosus are reviewed." @default.
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• W2000878181 date "2012-09-01" @default.
• W2000878181 modified "2023-10-17" @default.
• W2000878181 title "Microbial Metalloproteomes Explored Using MIRAGE" @default.
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• W2000878181 doi "https://doi.org/10.1002/cbdv.201100412" @default.
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