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- W2000887097 abstract "Forty-two Lactobacillus helveticus strains isolated from two whey starter cultures and three cheeses were typed by multilocus restriction typing (MLRT), analyzing the restriction fragment-length polymorphism of PCR products generated from eight loci of housekeeping genes: β-galactosidase, lactose permease, UDP-galactose-4 epimerase, β-galactosidase transcriptional regulator, D-lactate dehydrogenase, peptidase N, stress-inducible protein and S-layer protein. MLRT analysis indicated wide strain heterogeneity. Strains were grouped according to their source of isolation. The low intra-species polymorphism detected in the 16S rRNA gene did not allow grouping of the strains with the same sensitivity reached by MLRT of protein-coding genes. This is the first demonstration of MLRT as a technique for the discrimination of Lb. helveticus strains; this technique is a promising tool to evaluate genetic diversity of related strains from different ecological niches." @default.
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- W2000887097 date "2007-04-01" @default.
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- W2000887097 title "Multilocus restriction typing: A tool for studying molecular diversity within Lactobacillus helveticus of dairy origin" @default.
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- W2000887097 doi "https://doi.org/10.1016/j.idairyj.2006.03.002" @default.
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