FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2000887954> ?p ?o ?g. }

• W2000887954 endingPage "7755" @default.
• W2000887954 startingPage "7747" @default.
• W2000887954 abstract "Serotonergic neurotransmission is modulated by the membrane-embedded serotonin transporter (SERT). SERT mediates the reuptake of serotonin into the presynaptic neurons. Conformational changes in SERT occur upon binding of ions and substrate and are crucial for translocation of serotonin across the membrane. Our understanding of these conformational changes is mainly based on crystal structures of a bacterial homolog in various conformations, derived homology models of eukaryotic neurotransmitter transporters, and substituted cysteine accessibility method of SERT. However, the dynamic changes that occur in the human SERT upon binding of ions, the translocation of substrate, and the role of cholesterol in this interplay are not fully elucidated. Here we show that serotonin induces a dualistic conformational response in SERT. We exploited the substituted cysteine scanning method under conditions that were sensitized to detect a more outward-facing conformation of SERT. We found a novel high affinity outward-facing conformational state of the human SERT induced by serotonin. The ionic requirements for this new conformational response to serotonin mirror the ionic requirements for translocation. Furthermore, we found that membrane cholesterol plays a role in the dualistic conformational response in SERT induced by serotonin. Our results indicate the existence of a subpopulation of SERT responding differently to serotonin binding than hitherto believed and that membrane cholesterol plays a role in this subpopulation of SERT." @default.
• W2000887954 created "2016-06-24" @default.
• W2000887954 creator A5011324042 @default.
• W2000887954 creator A5021314904 @default.
• W2000887954 creator A5046032429 @default.
• W2000887954 creator A5052146012 @default.
• W2000887954 creator A5066211104 @default.
• W2000887954 date "2015-03-01" @default.
• W2000887954 modified "2023-10-17" @default.
• W2000887954 title "A Dualistic Conformational Response to Substrate Binding in the Human Serotonin Transporter Reveals a High Affinity State for Serotonin" @default.
• W2000887954 cites W1549115605 @default.
• W2000887954 cites W1592442520 @default.
• W2000887954 cites W1598676150 @default.
• W2000887954 cites W1964628514 @default.
• W2000887954 cites W1964672651 @default.
• W2000887954 cites W1969174524 @default.
• W2000887954 cites W1970870670 @default.
• W2000887954 cites W1972632823 @default.
• W2000887954 cites W1982053398 @default.
• W2000887954 cites W1982462017 @default.
• W2000887954 cites W1983636667 @default.
• W2000887954 cites W1985176332 @default.
• W2000887954 cites W1985179998 @default.
• W2000887954 cites W1985677418 @default.
• W2000887954 cites W1997460720 @default.
• W2000887954 cites W2000904449 @default.
• W2000887954 cites W2001397838 @default.
• W2000887954 cites W2016532705 @default.
• W2000887954 cites W2020717114 @default.
• W2000887954 cites W2021521177 @default.
• W2000887954 cites W2024340541 @default.
• W2000887954 cites W2026746093 @default.
• W2000887954 cites W2030224254 @default.
• W2000887954 cites W2030825942 @default.
• W2000887954 cites W2033921021 @default.
• W2000887954 cites W2042285022 @default.
• W2000887954 cites W2042534309 @default.
• W2000887954 cites W2044811013 @default.
• W2000887954 cites W2045056309 @default.
• W2000887954 cites W2046376309 @default.
• W2000887954 cites W2046508842 @default.
• W2000887954 cites W2051918534 @default.
• W2000887954 cites W2056497347 @default.
• W2000887954 cites W2057327534 @default.
• W2000887954 cites W2066607176 @default.
• W2000887954 cites W2070430670 @default.
• W2000887954 cites W2073566204 @default.
• W2000887954 cites W2075986351 @default.
• W2000887954 cites W2077681127 @default.
• W2000887954 cites W2079507733 @default.
• W2000887954 cites W2084290194 @default.
• W2000887954 cites W2091260123 @default.
• W2000887954 cites W2091649030 @default.
• W2000887954 cites W2093551180 @default.
• W2000887954 cites W2096242757 @default.
• W2000887954 cites W2098511931 @default.
• W2000887954 cites W2100992207 @default.
• W2000887954 cites W2102965606 @default.
• W2000887954 cites W2104622728 @default.
• W2000887954 cites W2122565898 @default.
• W2000887954 cites W2411172929 @default.
• W2000887954 cites W4233693399 @default.
• W2000887954 doi "https://doi.org/10.1074/jbc.m114.573477" @default.
• W2000887954 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4367276" @default.
• W2000887954 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25614630" @default.
• W2000887954 hasPublicationYear "2015" @default.
• W2000887954 type Work @default.
• W2000887954 sameAs 2000887954 @default.
• W2000887954 citedByCount "24" @default.
• W2000887954 countsByYear W20008879542016 @default.
• W2000887954 countsByYear W20008879542017 @default.
• W2000887954 countsByYear W20008879542018 @default.
• W2000887954 countsByYear W20008879542019 @default.
• W2000887954 countsByYear W20008879542020 @default.
• W2000887954 countsByYear W20008879542021 @default.
• W2000887954 countsByYear W20008879542022 @default.
• W2000887954 countsByYear W20008879542023 @default.
• W2000887954 crossrefType "journal-article" @default.
• W2000887954 hasAuthorship W2000887954A5011324042 @default.
• W2000887954 hasAuthorship W2000887954A5021314904 @default.
• W2000887954 hasAuthorship W2000887954A5046032429 @default.
• W2000887954 hasAuthorship W2000887954A5052146012 @default.
• W2000887954 hasAuthorship W2000887954A5066211104 @default.
• W2000887954 hasBestOaLocation W20008879541 @default.
• W2000887954 hasConcept C104317684 @default.
• W2000887954 hasConcept C12554922 @default.
• W2000887954 hasConcept C149011108 @default.
• W2000887954 hasConcept C170493617 @default.
• W2000887954 hasConcept C181199279 @default.
• W2000887954 hasConcept C185592680 @default.
• W2000887954 hasConcept C18903297 @default.
• W2000887954 hasConcept C2775864247 @default.
• W2000887954 hasConcept C2777289219 @default.
• W2000887954 hasConcept C2778897192 @default.
• W2000887954 hasConcept C2908747647 @default.
• W2000887954 hasConcept C2994592520 @default.
• W2000887954 hasConcept C55493867 @default.
• W2000887954 hasConcept C71240020 @default.

• next