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- W2000890664 abstract "(NH4)2SO4 precipitation and cellulose phosphate chromatography, commonly used to prepare plasma Factor V, have been found to produce degradation products of lower molecular weight and higher specific activity than the native protein. The so-called forms L and C (G. Philip, J. Moran and R. W. Colman, Biochemistry, 9 (1970) 2212) were shown not to be constituents of native Factor V but instead artifacts of preparative procedures. The inclusion of Ca2+ in the eluting buffer during gel filtration of bovine plasma Factor V allowed retention of most of the biological activity generally lost by this procedure. Concentration of Ca2+ higher than 0.001 M depressed the activity of factor V as measured in the one-stage assay." @default.
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- W2000890664 date "1972-02-01" @default.
- W2000890664 modified "2023-10-09" @default.
- W2000890664 title "Studies on the stability of Bovine plasma factor V" @default.
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- W2000890664 doi "https://doi.org/10.1016/0304-4165(72)90070-0" @default.
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