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- W2000896819 abstract "A simple procedure is described to maintain Chloroflexus cultures for relatively long times without frequent subculturing. All tested strains of Chloroflexus aurantiacus proved viable after more than 18 months of storage in dim light (100–200 lx) at 37–40°C as slow growing standing liquid cultures. During storage, no shaking, regular subculturing or transfer to fresh medium was required. To increase the span of generation time, cell survival and stability, various growth components and adsorption materials were screened. The anaerobic conditions, presence of sulfide and regular feeding with yeast extract favored prolonged viability during maintenance as liquid cultures. The presence of activated charcoal (0.05% w/v) in the media resulted in slowing down the growth rate and due to the absorption of harmful metabolites and waste products, the cells remained more stable and healthy during such maintenance. The cultures of Chloroflexus aggregans also survived several months under such conditions. Due to the use of moderate conditions, regrowth in fresh medium resulted within relatively short times with full pigmentation, indicating that the maintenance and cultivation coditions were optimal for photoautotrophic growth. The method is more suitable, effective and convenient than cryopreservation or lyophilization as the maintained cell suspensions can provide a continuous source of inocula, from the same batch of cell suspension, for routine work." @default.
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- W2000896819 date "1996-11-01" @default.
- W2000896819 modified "2023-10-12" @default.
- W2000896819 title "A convenient method for maintaining Chloroflexus for long time periods as slow growing liquid cultures" @default.
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- W2000896819 doi "https://doi.org/10.1016/s0167-7012(96)00942-6" @default.
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