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- W2000897659 abstract "SUMMARY A systematic study has been performed using a series of differentiated rat thyroid epithelial cell lines either uninfected or infected with Kirsten murine sarcoma virus (KiMSV), to determine the levels of the p21 product of the v-ras-Ki oncogene in transformed and normal cell lines. The p21 levels have been assayed by SDS-polyacrylamide gel electrophoresis of immunoprecipitates of 35S-labelled cell extracts and by a GDP binding assay. All cell lines analysed showed a significant increase in the levels of p21 after transformation with KiMSV compared to the p21 levels of uninfected and untransformed differentiated thyroid cells. The results reported here confirm the potential ability of the v-ras-Ki oncogene product to transform epithelial cells. They show, furthermore, that not only is p21 present in some epithelial cells transformed by KiMSV, but also that it is functionally active, as has been shown for fibroblasts transformed by the same virus, and that its functioning is maintained after passaging in vivo of the transformed cells." @default.
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- W2000897659 date "1984-11-01" @default.
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- W2000897659 title "Expression of the onc Gene of the Kirsten Murine Sarcoma Virus in Differentiated Rat Thyroid Epithelial Cell Lines" @default.
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- W2000897659 doi "https://doi.org/10.1099/0022-1317-65-11-1955" @default.
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