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- W2000901085 abstract "Intraperitoneal administration of mitomycin C (40 micrograms/100 g body weight) to male Wistar rats increased the ATPase activity in hypotonic extracts of liver cell nuclei for 4 days after injection. Partially purified ATPase, obtained by the DEAE-cellulose column chromatography of these extracts, showed a 14 times higher specific activity than that found in normal rat liver nuclei. The enzymatic activity was strongly enhanced by the addition of polynucleotides, especially poly A and poly I, to the assay mixture, but was inhibited by GTP, a chelating agent, heparin and thiol-group inhibitors. Quercetin and oligomycin were less effective, and ouabain showed no inhibitory effect. Mg2+ ions were essential, but neither Ca2+, Na+ nor K+ ions were required for the manifestation of the activity. These characteristic properties of the enzyme are similar to those of a nucleoside triphosphatase found in the nuclear matrix and envelope, suggesting that some energy-providing mechanisms involved in the repair processes of DNA damage or cellular injury are induced by mitomycin C administration." @default.
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- W2000901085 date "1989-12-01" @default.
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- W2000901085 title "Enhanced ATPase Activity in Liver Cell Nuclei Induced by Administration of Mitomycin C to Rats" @default.
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- W2000901085 doi "https://doi.org/10.1111/j.1349-7006.1989.tb01656.x" @default.
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