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- W2000901229 abstract "Summary In leaves of Nicotiana rustica the formation of ribosomal RNA is inhibited by FU at concentrations higher than 90 μg FU per g fresh weight.The synthesis of low molecular weight RNA and D-RNA is inhibited at concentrations more than 350μg FU per g fresh weight.The synthesis of nuclear DNA is also inhibited at lower FU-concentrations presumably via inhibition of thymidylate-synthetase. Application of 300μg FU/g fresh weight prevents the formation of cytoplasmic rRNA by interfering with the maturation process of the RNA.While high molecular weight RNA (mol.wt.> 1.4 × 10 6 D) slightly accumulates or remains unaffected after pretreatment of tobacco leaves with the antimetabolite, the formation of 1.4 × 10 6 D ribosomal pre-RNA and of mature cytoplasmic rRNA (mol.wt.of about 1.3 and 0.7 × 10 6 D) is almost completely inhibited.At the same concentration the formation of chloroplast ribosomal RNA, i.e.the synthesis of 1.28 and 0.7 × 10 6 D ribosomal pre-RNA and their transformation into mature rRNA remains unaffected by the drug. This different sensitivity of chloroplast and cytoplasmic rRNA formation to FU cannot be explained by a different uptake of the analogue into nuclei and chloroplasts, because labelled 5-fluorouracil is incorporated into cytoplasmic as well as into chloroplast ribosomal RNA." @default.
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- W2000901229 title "Effect of 5-Fluorouracil on the synthesis of chloroplast and cytoplasmic ribosomal RNA in leaves of Nicotiana rustica" @default.
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- W2000901229 doi "https://doi.org/10.1016/s0015-3796(80)80003-5" @default.
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