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- W2000902716 abstract "The assignment of the mass (m) value from the m/z value for ions with a multiple number of charges (z) in electrospray mass spectra usually utilizes multiple peaks of the same m but different z values, or unit-mass-separated isotopic peaks of the same z value from high resolution spectra. The latter approach is also feasible with much less resolving power using adduct ions of much higher mass separation. The application of this to mixture spectra containing many masses, such as spectra from tandem mass spectrometry (MS/MS) ion dissociation, does not appear to have been pointed out previously. Thus, replacing two protons by one Cu(2+) ion increases the mass by 61.5 Da, with this shift providing a mass scale for assignment of m and z from this pair of m/z values. The more common Na(+) adduct peaks provide a 22.0 Da separation, of utility for 1000 resolving power only below approximately 10 kDa. Further, collisional dissociation lowers the degree of Cu(2+) adduction in the resulting sequence-specific fragment ions much less than that of the corresponding Na(+) adducts, making the Cu(2+) adducts far more useful for m and z determination in MS/MS studies." @default.
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- W2000902716 date "1993-10-01" @default.
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- W2000902716 title "Mass and charge assignment for electrospray ions by cation adduction" @default.
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- W2000902716 doi "https://doi.org/10.1016/1044-0305(93)80041-v" @default.
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