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- W2000905982 abstract "Abstract A technique was developed for assessing effects of P on growth, in defined liquid media at orthophosphate concentrations comparable to those in soil solutions. Phosphate was supplied in sorbed form on iron oxide (goethite) separated from the liquid medium by a dialysis membrane. Depending on the level of sorbed P, concentrations in solution were established in the range 0.05 to 30 µ M . Analytical monitoring indicated that concentrations above 0.1 µ M were buffered against depletion by cell growth until populations exceeded 10 6 cells per ml. The system was used to compare P responses of seven strains of soybean and cowpea rhizobia grown from P‐depleted inocula at initial densities of 10 4 cells per ml. Two P‐efficient strains grew as rapidly at the lowest concentration (0.05 µ M ) as in a control medium at 2,000 µ M . They would seem unlikely to be P‐limited in the field. Other strains were less efficient. One strain could barely grow at P concentration below 1 µ M ; this strain could have difficulty colonizing P‐deficient soils or normal rhizospheres. Large differences in tolerance to low P among strains may be agronomically important. The technique could be simplified for routine screening of strains; and its operation at submicromolar concentrations could be improved for research on nutrition and requirements of P‐efficient rhizobia and other organisms." @default.
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- W2000905982 date "1981-05-01" @default.
- W2000905982 modified "2023-09-28" @default.
- W2000905982 title "Growth of Rhizobium Strains at Low Concentrations of Phosphate" @default.
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- W2000905982 doi "https://doi.org/10.2136/sssaj1981.03615995004500030016x" @default.
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